ArticlePflugers Archiv : European journal of physiology2008
Subunits alpha, beta and gamma of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A computational spatial whole-Cell model for hepatitis B viral infection and drug interactions.Scientific reports · 2023Article
- Cell death induction and protection by activation of ubiquitously expressed anion/cation channels. Part 3: the roles and properties of TRPM2 and TRPM7.Frontiers in cell and developmental biology · 2023Review
- Lysosomal solute and water transport.The Journal of cell biology · 2022Review
- Cation-Chloride Cotransporters, Na/K Pump, and Channels in Cell Water/Ionic Balance Regulation Under Hyperosmolar Conditions:Frontiers in cell and developmental biology · 2021Article
- Cell volume regulation in cancer cell migration driven by osmotic water flow.Cancer science · 2019Review
- Hypertonicity-induced cation channels in HepG2 cells: architecture and role in proliferation vs. apoptosis.The Journal of physiology · 2018Article
- Paracrine and autocrine regulation of gene expression by Wnt-inhibitor Dickkopf in wild-type and mutant hepatocytes.BMC systems biology · 2017Article
- Adaptive responses of cell hydration to a low temperature arrest.The Journal of physiology · 2016Article
- Hypotonic regulation of mouse epithelial sodium channel in Xenopus laevis oocytes.The Journal of membrane biology · 2013Article
- Effect of apical hyperosmotic sodium challenge and amiloride on sodium transport in human bronchial epithelial cells from cystic fibrosis donors.American journal of physiology. Cell physiology · 2013Article
- The ΔC splice-variant of TRPM2 is the hypertonicity-induced cation channel in HeLa cells, and the ecto-enzyme CD38 mediates its activation.The Journal of physiology · 2012Article
- Kinetics of hyperosmotically stimulated Na-K-2Cl cotransporter in Xenopus laevis oocytes.American journal of physiology. Cell physiology · 2011Article
- Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy.Pflugers Archiv : European journal of physiology · 2010Article
- alpha-ENaC is a functional element of the hypertonicity-induced cation channel in HepG2 cells and it mediates proliferation.Pflugers Archiv : European journal of physiology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
Specific small interfering RNA (siRNA) constructs were used to test for the functional relation of subunits alpha, beta, and gamma of the epithelial Na(+) channel (ENaC) to the hypertonicity-induced cation channel (HICC) in confluent rat hepatocytes. In current-clamp recordings, hypertonic stress (300 --> 400 mosM) increased membrane conductance from 75.4 +/- 9.4 to 91.1 +/- 11.2 pS (p < 0.001). The effect was completely blocked by 100 microM amiloride and reduced to 46, 30, and 45% of the control value by anti-alpha-, anti-beta-, and anti-gamma-rENaC siRNA, respectively. Scanning acoustic microscopy revealed an initial shrinkage of cells from 6.98 +/- 0.45 to 6.03 +/- 0.43 pl within 2 min. This passive response was then followed by a regulatory volume increase (RVI) by 0.42 +/- 0.05 pl (p < 0.001). With anti-alpha-, anti-beta-, and anti-gamma-rENaC siRNA, the volume response was reduced to 31, 31, and 36% of the reference level, respectively. It is concluded that all three subunits of the ENaC are functionally related to RVI and HICC activation in rat hepatocytes.
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