ArticlePloS one2007
Variable expression of Cre recombinase transgenes precludes reliable prediction of tissue-specific gene disruption by tail-biopsy genotyping.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- DSP and DPP are dispensable for initiation of dentin and enamel mineralization but critical for circumpulpal dentin mineralization.Scientific reports · 2025Article
- G9a inactivation in progenitor cells with Isl1-Cre with reduced recombinase activity models aspects of Dandy-Walker complex.Biology open · 2023Article
- Alternative splicing of METTL3 explains apparently METTL3-independent m6A modifications in mRNA.PLoS biology · 2022Article
- TheBiomedicines · 2020Article
- Analysis of Cre-mediated genetic deletion ofAmerican journal of physiology. Heart and circulatory physiology · 2019Article
- Cochlea-Specific Deletion of CaFrontiers in cellular neuroscience · 2019Article
- Considerations and guidelines for mouse metabolic phenotyping in diabetes research.Diabetologia · 2018Review
- Article
- Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis.The Journal of biological chemistry · 2014Article
- Mesangial cell αvβ8-integrin regulates glomerular capillary integrity and repair.American journal of physiology. Renal physiology · 2014Article
- Comparative analysis of the efficiency and specificity of myeloid-Cre deleting strains using ROSA-EYFP reporter mice.Journal of immunological methods · 2014Article
- Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.PloS one · 2013Article
- Promises and pitfalls of a Pannexin1 transgenic mouse line.Frontiers in pharmacology · 2013Article
- Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.The Journal of biological chemistry · 2012Article
- Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion.BMC physiology · 2012Article
- Immunomodulatory action of dietary fish oil and targeted deletion of intestinal epithelial cell PPARδ in inflammation-induced colon carcinogenesis.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- The mouse genetics toolkit: revealing function and mechanism.Genome biology · 2011Review
- Analysis of conditional gene deletion using probe based Real-Time PCR.BMC biotechnology · 2010Article
- Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice.The American journal of pathology · 2009Article
- Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs.PloS one · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing number of generations. This eventually led to the complete abrogation of gene expression of the inserted Cre cDNA while still being detectable at the genomic level. Conversely, loss of Cre expression caused an incomplete or even complete lack of disruption for the protein under investigation. As Cre expression in the tissue of interest in most cases cannot be addressed in vivo during the course of a study, our findings implicate the possibility that individual tail-biopsy genotypes may not necessarily indicate the presence or absence of gene disruption. This indicates that sustained post hoc analyses in regards to efficacy of disruption for every single study group member may be required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.