ArticlePloS one2007
Effect of differential N-linked and O-linked mannosylation on recognition of fungal antigens by dendritic cells.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Review
- Innate Pulmonary Phagocytes and Their Interactions with PathogenicJournal of fungi (Basel, Switzerland) · 2023Review
- Recent advances in antigen targeting to antigen-presenting cells in veterinary medicine.Frontiers in immunology · 2023Review
- Effect of O-linked glycosylation on the antigenicity, cellular uptake and trafficking in dendritic cells of recombinant Ber e 1.PloS one · 2021Article
- Hydroponic Treatment ofFrontiers in plant science · 2018Article
- N-glycosylation converts non-glycoproteins into mannose receptor ligands and reveals antigen-specific T cell responses in vivo.Oncotarget · 2017Article
- Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives.Critical reviews in biotechnology · 2016Review
- Exploiting fungal cell wall components in vaccines.Seminars in immunopathology · 2015Review
- Article
- Expression of Aspergillus nidulans phy gene in Nicotiana benthamiana produces active phytase with broad specificities.International journal of molecular sciences · 2014Article
- Dendritic cell immunotherapy: clinical outcomes.Clinical & translational immunology · 2014Review
- Fungal glycans and the innate immune recognition.Frontiers in cellular and infection microbiology · 2014Review
- Article
- Targeting antigens to dendritic cell receptors for vaccine development.Journal of drug delivery · 2013Review
- The role of dendritic cells in the innate recognition of pathogenic fungi (A. fumigatus, C. neoformans and C. albicans).Virulence · 2012Review
- Dendritic cells in antifungal immunity and vaccine design.Cell host & microbe · 2012Review
- CD4(+) T cell vaccination overcomes defective cross-presentation of fungal antigens in a mouse model of chronic granulomatous disease.The Journal of clinical investigation · 2012Article
- Isotope labeling in insect cells.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Immunity to fungal infections.Nature reviews. Immunology · 2011Review
- Recent advancements in cytotoxic T lymphocyte generation methods using carbohydrate-coated liposomes.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAn experimental approach for improving vaccine efficacy involves targeting antigens to mannose receptors (MRs) on dendritic cells (DCs) and other professional antigen presenting cells. Previously, we demonstrated that mannosylated Pichia pastoris-derived recombinant proteins exhibited increased immunogenicity compared to proteins lacking mannosylation. In order to gain insight into the mechanisms responsible for this observation, the present study examined the cellular uptake of the mannosylated and deglycosylated recombinant proteins. METHODOLOGY/PRINCIPAL
findingsUtilizing transfected cell lines, roles for the macrophage mannose receptor (MMR, CD206) and DC-SIGN (CD209) in the recognition of the mannosylated, but not deglycosylated, antigens were demonstrated. The uptake of mannosylated antigens into murine bone marrow-derived DCs (BMDCs) was inhibited by yeast mannans (YMs), suggesting a mannose-specific C-type lectin receptor-dependent process, while the uptake of deglycosylated antigens remained unaffected. In particular, antigens with both N-linked and extensive O-linked mannosylation showed the highest binding and uptake by BMDCs. Finally, confocal microscopy studies revealed that both mannosylated and deglycosylated P. pastoris-derived recombinant proteins localized in MHC class II+ compartments within BMDCs. CONCLUSIONS/SIGNIFICANCE: Taken together with our previous results, these data suggest that increased uptake by mannose-specific C-type lectin receptors is the major mechanism responsible for the enhanced antigenicity seen with mannosylated proteins. These findings have important implications for vaccine design and contribute to our understanding of how glycosylation affects the immune response to eukaryotic pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.