ArticleProtoplasma2007
Possible roles of actin and myosin during anaphase chromosome movements in locust spermatocytes.
Article in Protoplasma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Evidence of Non-microtubule Spindle Forces inFrontiers in molecular biosciences · 2020Article
- Gene expression changes elicited by a parasitic B chromosome in the grasshopper Eyprepocnemis plorans are consistent with its phenotypic effects.Chromosoma · 2019Article
- Multiple Pools of Nuclear Actin.Anatomical record (Hoboken, N.J. : 2007) · 2018Article
- Nuclear Actin: From Discovery to Function.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Gamma-actin is involved in regulating centrosome function and mitotic progression in cancer cells.Cell cycle (Georgetown, Tex.) · 2015Article
- And the dead shall rise: actin and myosin return to the spindle.Developmental cell · 2011Review
- Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- The molecular basis of anaphase A in animal cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the early Drosophila embryo.PloS one · 2011Article
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Article
- Identification of a kinase profile that predicts chromosome damage induced by small molecule kinase inhibitors.PLoS computational biology · 2009Article
- Abundance of actin filaments in the preprophase band and mitotic spindle of brick1 Zea mays mutant.Protoplasma · 2009Article
- Mitosis: spindle evolution and the matrix model.Protoplasma · 2009Article
- Myosin-10 and actin filaments are essential for mitotic spindle function.The Journal of cell biology · 2008Article
- What generates flux of tubulin in kinetochore microtubules?Protoplasma · 2008Article
- Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement.Cell & chromosome · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested whether the mechanisms of chromosome movement during anaphase in locust (Locusta migratoria L.) spermatocytes might be similar to those described for crane-fly spermatocytes. Actin and myosin have been implicated in anaphase chromosome movements in crane-fly spermatocytes, as indicated by the effects of inhibitors and by the localisations of actin and myosin in spindles. In this study, we tested whether locust spermatocyte spindles also utilise actin and myosin, and whether actin is involved in microtubule flux. Living locust spermatocytes were treated with inhibitors of actin (latrunculin B and cytochalasin D), myosin (BDM), or myosin phosphorylation (Y-27632 and ML-7). We added drugs (individually) during anaphase. Actin inhibitors alter anaphase: chromosomes either completely stop moving, slow, or sometimes accelerate. The myosin inhibitor, BDM, also alters anaphase: in most cases, the chromosomes drastically slow or stop. ML-7, an inhibitor of MLCK, causes chromosomes to stop, slow, or sometimes accelerate, similar to actin inhibitors. Y-27632, an inhibitor of Rho-kinase, drastically slows or stops anaphase chromosome movements. The effects of the drugs on anaphase movement are reversible: most of the half-bivalents resumed movement at normal speed after these drugs were washed out. Actin and myosin were present in the spindles in locations consistent with their possible involvement in force production. Microtubule flux along kinetochore fibres is an actin-dependent process, since LatB completely removes or drastically reduces the gap in microtubule acetylation at the kinetochore. These results suggest that actin and myosin are involved in anaphase chromosome movements in locust spermatocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.