ArticleEukaryotic cell2007
Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 105 citations in OpenAlex.
- Genetic background shapes the transcriptional activity and phenotypic contribution of a horizontally acquired region in yeast.Molecular biology and evolution · 2026Article
- Effects of global change drivers on the expression of pathogenicity and stress genes in dryland soil fungi.mSphere · 2024Article
- Quorum sensing: cell-to-cell communication inFrontiers in microbiology · 2023Review
- Genomic Adaptations ofMicroorganisms · 2022Review
- Variation in pH gradients and FLO11 expression in mat biofilms from environmental isolates of the yeast Saccharomyces cerevisiae.MicrobiologyOpen · 2022Article
- FLO11, a Developmental Gene Conferring Impressive Adaptive Plasticity to the YeastPathogens (Basel, Switzerland) · 2021Review
- The Flo Adhesin Family.Pathogens (Basel, Switzerland) · 2021Review
- Article
- Intragenic repeat expansion in the cell wall protein geneGenome research · 2020Article
- A re-evaluation of diastatic Saccharomyces cerevisiae strains and their role in brewing.Applied microbiology and biotechnology · 2020Review
- Variation at an adhesin locus suggests sociality in natural populations of the yeastProceedings. Biological sciences · 2019Article
- Adhesins of Yeasts: Protein Structure and Interactions.Journal of fungi (Basel, Switzerland) · 2018Review
- Synthetic gene regulation for independent external induction of theCommunications biology · 2018Article
- Article
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- The Origin of Animal Multicellularity and Cell Differentiation.Developmental cell · 2017Review
- Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.Microbial cell (Graz, Austria) · 2016Article
- Nutrient depletion modifies cell wall adsorption activity of wine yeast.World journal of microbiology & biotechnology · 2016Article
- Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival.mBio · 2015Article
- FLO11 Gene Is Involved in the Interaction of Flor Strains of Saccharomyces cerevisiae with a Biofilm-Promoting Synthetic Hexapeptide.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Flo11/Muc1 flocculin has diverse phenotypic effects. Saccharomyces cerevisiae cells of strain background Sigma1278b require Flo11p to form pseudohyphae, invade agar, adhere to plastic, and develop biofilms, but they do not flocculate. We show that S. cerevisiae var. diastaticus strains, on the other hand, exhibit Flo11-dependent flocculation and biofilm formation but do not invade agar or form pseudohyphae. In order to study the nature of the Flo11p proteins produced by these two types of strains, we examined secreted Flo11p, encoded by a plasmid-borne gene, in which the glycosylphosphatidylinositol anchor sequences had been replaced by a histidine tag. A protein of approximately 196 kDa was secreted from both strains, which upon purification and concentration, aggregated into a form with a very high molecular mass. When secreted Flo11p was covalently attached to microscopic beads, it conferred the ability to specifically bind to S. cerevisiae var. diastaticus cells, which flocculate, but not to Sigma1278b cells, which do not flocculate. This was true for the 196-kDa form as well as the high-molecular-weight form of Flo11p, regardless of the strain source. The coated beads bound to S. cerevisiae var. diastaticus cells expressing FLO11 and failed to bind to cells with a deletion of FLO11, demonstrating a homotypic adhesive mechanism. Flo11p was shown to be a mannoprotein. Bead-to-cell adhesion was inhibited by mannose, which also inhibits Flo11-dependent flocculation in vivo, further suggesting that this in vitro system is a useful model for the study of fungal adhesion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.