ArticleJournal of bacteriology2007
Comparative genomic hybridization detects secondary chromosomal deletions in Escherichia coli K-12 MG1655 mutants and highlights instability in the flhDC region.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- OP50, a bacterial strain conventionally used as food for laboratory maintenance ofmicroPublication biology · 2020Article
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- Factors that affect transfer of the IncI1 β-lactam resistance plasmid pESBL-283 between E. coli strains.PloS one · 2015Article
- Essential validation methods for E. coli strains created by chromosome engineering.Journal of biological engineering · 2015Article
- Genome dynamics during experimental evolution.Nature reviews. Genetics · 2013Review
- Isolation of generalized transducing bacteriophages for uropathogenic strains of Escherichia coli.Applied and environmental microbiology · 2011Article
- Genotype and phenotypes of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization.Infection and immunity · 2011Article
- YieJ (CbrC) mediates CreBC-dependent colicin E2 tolerance in Escherichia coli.Journal of bacteriology · 2010Article
- Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains.BMC microbiology · 2009Article
- The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.PloS one · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in delta fnr, delta crp, and delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that nonmotile progeny are found in some MG1655 directed deletion mutants, and studies on the effects of gene knockouts should be viewed with caution when the mutants have not been screened for the presence of secondary deletions or confirmed by other methods.
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Registered trials
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