ArticleJournal of bacteriology2007
Evolution and functional characterization of the RH50 gene from the ammonia-oxidizing bacterium Nitrosomonas europaea.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Evidence of habitat specificity in sponge microbiomes from Antarctica.Environmental microbiome · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- Nitrification and beyond: metabolic versatility of ammonia oxidising archaea.The ISME journal · 2023Review
- Prokaryotic ammonium transporters: what has three decades of research revealed?Microbiology (Reading, England) · 2023Article
- Article
- Horizontal gene transfer drives the evolution of Rh50 permeases in prokaryotes.BMC evolutionary biology · 2017Article
- What drives the occurrence of the melioidosis bacterium Burkholderia pseudomallei in domestic gardens?PLoS neglected tropical diseases · 2015Article
- Article
- Role of NH3 and NH4+ transporters in renal acid-base transport.American journal of physiology. Renal physiology · 2011Review
- Evidence for different contributions of archaea and bacteria to the ammonia-oxidizing potential of diverse Oregon soils.Applied and environmental microbiology · 2010Article
- The Rh protein family: gene evolution, membrane biology, and disease association.Cellular and molecular life sciences : CMLS · 2010Review
- The 1.3-A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The family of ammonia and ammonium channel proteins comprises the Amt proteins, which are present in all three domains of life with the notable exception of vertebrates, and the homologous Rh proteins (Rh50 and Rh30) that have been described thus far only in eukaryotes. The existence of an RH50 gene in bacteria was first revealed by the genome sequencing of the ammonia-oxidizing bacterium Nitrosomonas europaea. Here we have used a phylogenetic approach to study the evolution of the N. europaea RH50 gene, and we show that this gene, probably as a component of an integron cassette, has been transferred to the N. europaea genome by horizontal gene transfer. In addition, by functionally characterizing the Rh50(Ne) protein and the corresponding knockout mutant, we determined that NeRh50 can mediate ammonium uptake. The RH50(Ne) gene may thus have replaced functionally the AMT gene, which is missing in the genome of N. europaea and may be regarded as a case of nonorthologous gene displacement.
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Registered trials
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