ArticleApplied and environmental microbiology2007
Expanding the Helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 52 citations in OpenAlex.
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- Helicobacter pylori-mediated gastric pathogenesis is attenuated by treatment of 2-deoxyglucose and metformin.Journal of microbiology (Seoul, Korea) · 2022Article
- Characterization of East-Asian Helicobacter pylori encoding Western EPIYA-ABC CagA.Journal of microbiology (Seoul, Korea) · 2022Article
- Geographic diversity in Helicobacter pylori oipA genotype between Korean and United States isolates.Journal of microbiology (Seoul, Korea) · 2021Article
- Evolutionary mechanism leading to the multi-cagA genotype in Helicobacter pylori.Scientific reports · 2019Article
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- ArsRS-Dependent Regulation ofFrontiers in microbiology · 2018Article
- Methylomic and phenotypic analysis of the ModH5 phasevarion of Helicobacter pylori.Scientific reports · 2017Article
- Functional disruption of peroxiredoxin by bismuth antiulcer drugs attenuates Helicobacter pylori survival.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Article
- N-acetylcysteine prevents the development of gastritis induced by Helicobacter pylori infection.Journal of microbiology (Seoul, Korea) · 2017Article
- Cot kinase plays a critical role in Helicobacter pylori-induced IL-8 expression.Journal of microbiology (Seoul, Korea) · 2017Article
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- Structure-function analyses of metal-binding sites of HypA reveal residues important for hydrogenase maturation in Helicobacter pylori.PloS one · 2017Article
- Article
- Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Helicobacter pylori is an important human pathogen. However, the study of this organism is often limited by a relative shortage of genetic tools. In an effort to expand the methods available for genetic study, an endogenous H. pylori plasmid was modified for use as a transcriptional reporter and as a complementation vector. This was accomplished by addition of an Escherichia coli origin of replication, a kanamycin resistance cassette, a promoterless gfpmut3 gene, and a functional multiple cloning site to form pTM117. The promoters of amiE and pfr, two well-characterized Fur-regulated promoters, were fused to the promoterless gfpmut3, and green fluorescent protein (GFP) expression of the fusions in wild-type and delta fur strains was analyzed by flow cytometry under iron-replete and iron-depleted conditions. GFP expression was altered as expected based on current knowledge of Fur regulation of these promoters. RNase protection assays were used to determine the ability of this plasmid to serve as a complementation vector by analyzing amiE, pfr, and fur expression in wild-type and delta fur strains carrying a wild-type copy of fur on the plasmid. Proper regulation of these genes was restored in the delta fur background under high- and low-iron conditions, signifying complementation of both iron-bound and apo Fur regulation. These studies show the potential of pTM117 as a molecular tool for genetic analysis of H. pylori.
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Registered trials
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