ArticleBiophysical journal2008
Diffusion of glycerol through Escherichia coli aquaglyceroporin GlpF.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 106 citations in OpenAlex.
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- Free energy and kinetics of cAMP permeation through connexin26 via applied voltage and milestoning.Biophysical journal · 2021Article
- In Silico Prediction of Permeability Coefficients.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Free Energy Landscape and Conformational Kinetics of Hoogsteen Base Pairing in DNA vs. RNA.Biophysical journal · 2020Article
- Aquaporin-7: A Dynamic Aquaglyceroporin With Greater Water and Glycerol Permeability Than Its Bacterial Homolog GlpF.Frontiers in physiology · 2020Article
- Transparent polyvinyl-alcohol cryogel as immobilisation matrix for continuous biohydrogen production by phototrophic bacteria.Biotechnology for biofuels · 2020Article
- Water channel pore size determines exclusion properties but not solute selectivity.Scientific reports · 2019Article
- Glycerol transport through the aquaglyceroporin GlpF: bridging dynamics and kinetics with atomic simulation.Chemical science · 2019Article
- Atomistic Analysis of ToxN and ToxI Complex Unbinding Mechanism.International journal of molecular sciences · 2018Article
- Selective Permeability of Carboxysome Shell Pores to Anionic Molecules.The journal of physical chemistry. B · 2018Article
- Permeability and ammonia selectivity in aquaporin TIP2;1: linking structure to function.Scientific reports · 2018Article
- Insights Into Arsenite and Arsenate Uptake Pathways Using a Whole Cell Biosensor.Frontiers in microbiology · 2018Article
- Human Aquaporin 4 Gating Dynamics under Perpendicularly-Oriented Electric-Field Impulses: A Molecular Dynamics Study.International journal of molecular sciences · 2016Article
- Computational Studies of Molecular Permeation through Connexin26 Channels.Biophysical journal · 2016Article
- Revealing the binding modes and the unbinding of 14-3-3σ proteins and inhibitors by computational methods.Scientific reports · 2015Article
- Enhanced sampling techniques in molecular dynamics simulations of biological systems.Biochimica et biophysica acta · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The glycerol uptake facilitator, GlpF, a major intrinsic protein found in Escherichia coli, selectively conducts water and glycerol across the inner membrane. The free energy landscape characterizing the assisted transport of glycerol by this homotetrameric aquaglyceroporin has been explored by means of equilibrium molecular dynamics over a timescale spanning 0.12 micros. To overcome the free energy barriers of the conduction pathway, an adaptive biasing force is applied to the glycerol molecule confined in each of the four channels. The results illuminate the critical role played by intramolecular relaxation on the diffusion properties of the permeant. These free energy calculations reveal that glycerol tumbles and isomerizes on a timescale comparable to that spanned by its adaptive-biasing-force-assisted conduction in GlpF. As a result, reorientation and conformational equilibrium of glycerol in GlpF constitute a bottleneck in the molecular simulations of the permeation event. A profile characterizing the position-dependent diffusion of the permeant has been determined, allowing reaction rate theory to be applied for investigating conduction kinetics based on the measured free energy landscape.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.