ArticleAnalytical biochemistry2007
Alkaline methanolysis of lipid extracts extends shotgun lipidomics analyses to the low-abundance regime of cellular sphingolipids.
Article in Analytical biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 96 citations in OpenAlex.
- A Sphingolipidomic Profiling Approach for Comparing X-ray-Exposed and Unexposed HepG2 Cells.International journal of molecular sciences · 2023Article
- Advances in Analyzing the Breast Cancer Lipidome and Its Relevance to Disease Progression and Treatment.Journal of mammary gland biology and neoplasia · 2021Review
- HIGH RESOLUTION MASS SPECTROMETRY IN LIPIDOMICS.Mass spectrometry reviews · 2021Review
- Comprehensive Identification of Glycosphingolipids in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry.Metabolites · 2021Article
- Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection.Journal of the American Society for Mass Spectrometry · 2021Article
- Mass Spectrometry-Based Shotgun Lipidomics for Cancer Research.Advances in experimental medicine and biology · 2021Article
- Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.Analytica chimica acta · 2020Review
- An Innovative Lipidomic Workflow to Investigate the Lipid Profile in a Cystic Fibrosis Cell Line.Cells · 2020Article
- Lipidomics from sample preparation to data analysis: a primer.Analytical and bioanalytical chemistry · 2020Review
- Sphingolipidomics in Translational Sepsis Research-Biomedical Considerations and Perspectives.Frontiers in medicine · 2020Review
- Tutorial on lipidomics.Analytica chimica acta · 2019Review
- A Targeted Mass Spectrometric Analysis Reveals the Presence of a Reduced but Dynamic Sphingolipid Metabolic Pathway in an Ancient Protozoan,Frontiers in cellular and infection microbiology · 2019Article
- Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why?Mass spectrometry reviews · 2017Review
- Lipidomics: Techniques, Applications, and Outcomes Related to Biomedical Sciences.Trends in biochemical sciences · 2016Review
- Shotgun Lipidomics Approach to Stabilize the Regiospecificity of Monoglycerides Using a Facile Low-Temperature Derivatization Enabling Their Definitive Identification and Quantitation.Analytical chemistry · 2016Article
- Advanced Shotgun Lipidomics for Characterization of Altered Lipid Patterns in Neurodegenerative Diseases and Brain Injury.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Novel advances in shotgun lipidomics for biology and medicine.Progress in lipid research · 2016Review
- Alterations in mouse brain lipidome after disruption of CST gene: a lipidomics study.Molecular neurobiology · 2014Article
- Development of an automated multi-injection shotgun lipidomics approach using a triple quadrupole mass spectrometer.Lipids · 2014Article
- Multidimensional mass spectrometry-based shotgun lipidomics.Methods in molecular biology (Clifton, N.J.) · 2014Article
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5 authors at 1 institution in 1 country.
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Abstract
Sphingolipids that contain a sphingoid base are composed of hundreds to thousands of distinct compounds, many of which serve as lipid regulators of biological functions. The global analysis of the large number of low-abundance sphingolipid molecular species has been hampered in many cases by the sphingolipid molecular species being overwhelmed by the quantity of other classes of lipid (e.g., glycerophospholipid) molecular species present, thereby imposing severe restrictions on the dynamic range of their measurement using shotgun lipidomics. Herein, we developed a facile approach in which the sphingolipids of cellular extracts were dramatically enriched by direct alkaline methanolysis of lipid extracts followed by extraction to remove the large majority of other endogenous lipid classes. Through direct infusion of the resultant enriched solution, we identified and quantitated a variety of very-low-abundance sphingolipid classes (e.g., sphingosine, psychosine, and lysosphingomyelin) and molecular species (e.g., sphingomyelin) using electrospray ionization mass spectrometry (i.e., shotgun sphingolipidomics). Accordingly, through utilization of these facile enrichment techniques, direct penetrance into the sphingolipidomes has been greatly extended, facilitating new insights into their metabolism and signaling functions in biological systems.
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