ArticleJournal of molecular biology2007
Site-directed alkylation of LacY: effect of the proton electrochemical gradient.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- The role of TMS 12 in the staphylococcal multidrug efflux protein QacA.The Journal of antimicrobial chemotherapy · 2023Article
- Fluorescent Binding Protein Sensors for Detection and Quantification of Biochemicals, Metabolites, and Natural Products.Bio-protocol · 2022Article
- Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.Chemical reviews · 2021Review
- Site-Directed Alkylation Detected by In-Gel Fluorescence (SDAF) to Determine the Topology Map and Probe the Solvent Accessibility of Membrane Proteins.Scientific reports · 2019Article
- It takes two to tango: The dance of the permease.The Journal of general physiology · 2019Review
- Dynamic distinctions in the NaThe Journal of biological chemistry · 2017Article
- Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.Biochemistry · 2016Article
- A chemiosmotic mechanism of symport.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Real-time conformational changes in LacY.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The periplasmic cavity of LacY mutant Cys154→Gly: how open is open?Biochemistry · 2013Article
- Trp replacements for tightly interacting Gly-Gly pairs in LacY stabilize an outward-facing conformation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Role of the irreplaceable residues in the LacY alternating access mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Evidence for an intermediate conformational state of LacY.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Lactose permease and the alternating access mechanism.Biochemistry · 2011Article
- Opening the periplasmic cavity in lactose permease is the limiting step for sugar binding.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- An early event in the transport mechanism of LacY protein: interaction between helices V and I.The Journal of biological chemistry · 2011Article
- Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.Biochemistry · 2011Article
- The alternating access transport mechanism in LacY.The Journal of membrane biology · 2011Review
- Probing the periplasmic-open state of lactose permease in response to sugar binding and proton translocation.Journal of molecular biology · 2010Article
- Delineating electrogenic reactions during lactose/H+ symport.Biochemistry · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous N-ethylmaleimide-labeling studies show that ligand binding increases the reactivity of single-Cys mutants located predominantly on the periplasmic side of LacY and decreases reactivity of mutants located for the most part of the cytoplasmic side. Thus, sugar binding appears to induce opening of a periplasmic pathway with closing of the cytoplasmic cavity resulting in alternative access of the sugar-binding site to either side of the membrane. Here we describe the use of a fluorescent alkylating reagent that reproduces the previous observations with respect to sugar binding. We then show that generation of an H(+) electrochemical gradient (Delta(mu (H)+), interior negative) increases the reactivity of single-Cys mutants on the periplasmic side of the sugar-binding site and in the putative hydrophilic pathway. The results suggest that Delta(mu (H)+), like sugar, acts to increase the probability of opening on the periplasmic side of LacY.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.