ArticleActa biomaterialia2008
Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes.
Article in Acta biomaterialia, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- Differential pulse voltammetry and chronoamperometry as analytical tools for epinephrine detection using a tyrosinase-based electrochemical biosensor.RSC advances · 2022Article
- Topography-Mediated Enhancement of Nonviral Gene Delivery in Stem Cells.Pharmaceutics · 2022Article
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes.Frontiers in chemistry · 2019Article
- Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors.Trends in biotechnology · 2016Review
- MicroRNA delivery for regenerative medicine.Advanced drug delivery reviews · 2015Review
- Vascular gene transfer from metallic stent surfaces using adenoviral vectors tethered through hydrolysable cross-linkers.Journal of visualized experiments : JoVE · 2014Article
- High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts.Biomaterials · 2011Article
- Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.Nano today · 2010Article
- Engineering surfaces for substrate-mediated gene delivery using recombinant proteins.Biomacromolecules · 2009Article
- Fibrin hydrogels for non-viral vector delivery in vitro.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios.Acta biomaterialia · 2009Article
- Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery.Biotechnology and bioengineering · 2009Article
- Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury.Biomaterials · 2009Article
- Self-assembling peptide-lipoplexes for substrate-mediated gene delivery.Acta biomaterialia · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG- and carboxylic acid-terminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.