ArticleNucleic acids research2007
Discovery of a mRNA mitochondrial localization element in Saccharomyces cerevisiae by nonhomologous random recombination and in vivo selection.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Rapid discovery of functional RNA domains.Nucleic acids research · 2025Article
- Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish.Life science alliance · 2023Article
- The Mitochondrial Genome in Aging and Disease and the Future of Mitochondrial Therapeutics.Biomedicines · 2022Review
- RNA localization regulates diverse and dynamic cellular processes.Traffic (Copenhagen, Denmark) · 2018Review
- Genomic analysis of RNA localization.RNA biology · 2014Review
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- Localization of mRNAs coding for mitochondrial proteins in the yeast Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- Conservation defines functional motifs in the squint/nodal-related 1 RNA dorsal localization element.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
In budding yeast, over 100 nuclear-encoded mRNAs are localized to the mitochondria. The determinants of mRNA localization to the mitochondria are not well understood, and protein factors involved in this process have not yet been identified. To reveal the sequence determinants for mitochondrial localization in a comprehensive and unbiased manner, we generated highly diversified libraries of 3' UTR regions from a known mitochondrially localized mRNA by nonhomologous random recombination (NRR) and subjected the resulting sequences to an in vivo selection that links cell survival to mitochondrial mRNA localization. When applied to the yeast ATP2 mRNA, this approach rapidly identified a 50-nt consensus motif, designated Min2, as well as two Min2-homologous regions naturally present downstream of the ATP2 stop codon, which are sufficient when appended to the 3' end of various reporter mRNAs to induce mitochondrial localization. Site-directed mutagenesis of Min2 revealed primary and secondary structure elements that contribute to localization activity. In addition, the Min2 motif may facilitate the identification of proteins involved in this mode of establishing cellular asymmetry.
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