ArticleBMC genomics2007
Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 4 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 4 syntheses or guidelines pooled it, 63 citations in OpenAlex.
- A common gene signature across multiple studies relate biomarkers and functional regulation in tolerance to renal allograft.Kidney international · 2015Pooled it
- Meta-Analysis of Microarray Data of Rainbow Trout Fry Gonad Differentiation Modulated by Ethynylestradiol.PloS one · 2015Pooled it
- Transcriptional assessment by microarray analysis and large-scale meta-analysis of the metabolic capacity of cardiac and skeletal muscle tissues to cope with reduced nutrient availability in Gilthead Sea Bream (Sparus aurata L.).Marine biotechnology (New York, N.Y.) · 2014Pooled it
- Meta-analysis of muscle transcriptome data using the MADMuscle database reveals biologically relevant gene patterns.BMC genomics · 2011Pooled it
- Dietary pine pollen induces masculinization in Nile tilapia (Oreochromis niloticus, L. 1758) by modulating sex-biased gene expression and steroid hormone profiles.Biology of sex differences · 2026Article
- Gonadal transdifferentiation in teleosts during normal and altered physiology-a review.Fish physiology and biochemistry · 2026Review
- Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish.International journal of molecular sciences · 2021Review
- Article
- Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.BMC genomics · 2017Article
- Morphology, sex steroid level and gene expression analysis in gonadal sex reversal of triploid female (XXX) rainbow trout (Oncorhynchus mykiss).Fish physiology and biochemistry · 2016Article
- Identification of conserved hepatic transcriptomic responses to 17β-estradiol using high-throughput sequencing in brown trout.Physiological genomics · 2015Article
- Adaptation of a Bioinformatics Microarray Analysis Workflow for a Toxicogenomic Study in Rainbow Trout.PloS one · 2015Article
- Profiling of androgen response in rainbow trout pubertal testis: relevance to male gonad development and spermatogenesis.PloS one · 2013Article
- Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes.PloS one · 2013Article
- Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation.Marine biotechnology (New York, N.Y.) · 2012Review
- Immune response and mitochondrial metabolism are commonly deregulated in DMD and aging skeletal muscle.PloS one · 2011Article
- Article
- Studies on feminization, sex determination, and differentiation of the Southern catfish, Silurus meridionalis--a review.Fish physiology and biochemistry · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFish gonadal sex differentiation is affected by sex steroids treatments providing an efficient strategy to control the sexual phenotype of fish for aquaculture purposes. However, the biological effects of such treatments are poorly understood. The aim of this study was to identify the main effects of an androgen masculinizing treatment (11beta-hydroxyandrostenedione, 11betaOHDelta4, 10 mg/kg of food for 3 months) on gonadal gene expression profiles of an all-female genetic population of trout. To characterize the most important molecular features of this process, we used a large scale gene expression profiling approach using rainbow trout DNA microarrays combined with a detailed gene ontology (GO) analysis.
results2,474 genes were characterized as up-regulated or down-regulated in trout female gonads masculinized by androgen in comparison with control male or female gonads from untreated all-male and all-female genetic populations. These genes were classified in 13 k-means clusters of temporally correlated expression profiles. Gene ontology (GO) data mining revealed that androgen treatment triggers a marked down-regulation of genes potentially involved in early oogenesis processes (GO 'mitotic cell cycle', 'nucleolus'), an up-regulation of the translation machinery (GO 'ribosome') along with a down-regulation of proteolysis (GO 'proteolysis', 'peptidase' and 'metallopeptidase activity'). Genes considered as muscle fibres markers (GO 'muscle contraction') and genes annotated as structural constituents of the extracellular matrix (GO 'extracellular matrix') or related to meiosis (GO 'chromosome' and 'meiosis') were found significantly enriched in the two clusters of genes specifically up-regulated in androgen-treated female gonads. GO annotations 'Sex differentiation' and 'steroid biosynthesis' were enriched in a cluster of genes with high expression levels only in control males. Interestingly none of these genes were stimulated by the masculinizing androgen treatment.
conclusionThis study provides evidence that androgen masculinization results in a marked dysregulation of early gene expression profiles when compared to natural testicular or ovarian differentiation. Based on these results we suggest that, in our experimental conditions, androgen masculinization proceeds mainly through an early inhibition of female development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.