ArticleCell stress & chaperones2007
Expression of heat shock protein 70 in the thermally stressed antarctic clam Laternula elliptica.
Article in Cell stress & chaperones, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- The Role of the Heat Shock Cognate Protein 70 Genes in Sex Determination and Differentiation of Chinese Tongue Sole (International journal of molecular sciences · 2023Article
- Expression profiling of HSP 70 and interleukins 2, 6 and 12 genes of Barki sheep during summer and winter seasons in two different locations.International journal of biometeorology · 2022Article
- Comparative Transcriptomics of the Northern Quahog Mercenaria mercenaria and Southern Quahog Mercenaria campechiensis in Response to Chronic Heat Stress.Marine biotechnology (New York, N.Y.) · 2022Article
- Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam.Cell stress & chaperones · 2020Article
- Association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in Indian sheep (Ovis aries).Cell stress & chaperones · 2017Article
- Cloning and expression analysis of HSP70 gene from mangrove plant Kandelia obovata under cold stress.Ecotoxicology (London, England) · 2015Article
- Hsp transcript induction is correlated with physiological changes under drought stress in Indian mustard.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Oxidative stress, heats shock protein and histopathological effects in the gills of African catfish, Clarias gariepinus induced by bridge runoffs.Environmental monitoring and assessment · 2015Article
- Article
- Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses.Fish physiology and biochemistry · 2014Review
- Article
- Age-dependent expression of stress and antimicrobial genes in the hemocytes and siphon tissue of the Antarctic bivalve, Laternula elliptica, exposed to injury and starvation.Cell stress & chaperones · 2014Article
- The association of SNPs in Hsp90β gene 5' flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds.Molecular biology reports · 2013Article
- Molecular characterization of three heat shock protein 70 genes and their expression profiles under thermal stress in the citrus red mite.Molecular biology reports · 2012Article
- Ocean acidification at high latitudes: potential effects on functioning of the Antarctic bivalve Laternula elliptica.PloS one · 2011Article
- Molecular characterization and induction of heat shock protein 90 in the Antarctic bivalve Laternula elliptica.Cell stress & chaperones · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock protein 70 (designated Laternula elliptica Hsp70 (LEHsp70)) expression was investigated in an Antarctic mud clam to see whether or not the inducible heat shock response has been conserved throughout over 25 million years of adaptation to constant low environmental temperatures. LEHsp70 cDNA was cloned and sequenced from the Antarctic clam Laternula elliptica. We used degenerated primers designed in the highly conserved regions of Hsp to amplify the corresponding mRNA, and full-length cDNA was obtained by rapid amplification of cDNA ends (RACE). The full length of LEHsp70 cDNA was 2470 bp, with a 5' untranslated region (UTR) of 92 bp, a 3' UTR of 416 bp, and an open reading frame (ORF) of 1962 bp encoding a polypeptide of 653 amino acids with an estimated molecular mass of 71.266 kDa and an estimated isoelectric point of 5.20. LEHsp70 contained highly conserved functional motifs of the cytosolic Hsp70 family. Expression of the LEHsp70 gene was quantified by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) of digestive gland and gill tissues. Heat shock (10 degrees C for different time periods) caused rapid induction of LEHsp70. A significant 4.6 +/- 0.14-fold increase in the LEHsp70/beta-Actin mRNA ratio occurred in the gill at 12 hours, which returned to baseline after 48 hours. In contrast, the maximum expression in the digestive gland (3.6 +/- 0.36) was reached at 24 hours and was still significant after 48 hours (1.89 +/- 0.21). This indicates that LEHsp70 may play an important role in mediating thermal stress and tolerance in this clam.
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