ArticleGenome biology2007
Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 136 citations in OpenAlex.
- ARABIDOPSIS Bsister and SEEDSTICK MADS-box transcription factors modulate maternal nutrient flow for seed development in Arabidopsis.Nature communications · 2026Article
- The post-fertilization archegonium gene network of seedless plants contributed to the origin of seeds.Nature communications · 2026Article
- VPS13 has an important role in female germline development in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Differential gene expression analysis identifies a group of defensin-like peptides from Solanum chacoense ovules with in vitro pollen tube attraction activity.Plant molecular biology · 2025Article
- Embryo sac cellularization defects lead to supernumerary egg cells and twin embryos iniScience · 2024Article
- Spatiotemporal control of miR398 biogenesis, via chromatin remodeling and kinase signaling, ensures proper ovule development.The Plant cell · 2021Article
- The Importance of Cytokinins during Reproductive Development in Arabidopsis and Beyond.International journal of molecular sciences · 2020Review
- Floral transcriptomes reveal gene networks in pineapple floral growth and fruit development.Communications biology · 2020Article
- Comparative gene expression profile analysis of ovules provides insights into Jatropha curcas L. ovule development.Scientific reports · 2019Article
- A brief note on genes that trigger components of apomixis.Journal of biosciences · 2019Review
- Characterization of filament-forming CTP synthases from Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2018Article
- Genome-Wide Analysis of DNA Methylation During Ovule Development of Female-Sterile RiceG3 (Bethesda, Md.) · 2017Article
- The Molecular Dialog between Flowering Plant Reproductive Partners Defined by SNP-Informed RNA-Sequencing.The Plant cell · 2017Article
- Patterning the Female Gametophyte of Flowering Plants.Plant physiology · 2017Review
- Transcriptome Analysis of a Female-sterile Mutant (Frontiers in plant science · 2017Article
- SUPPRESSOR OF FRIGIDA (SUF4) Supports Gamete Fusion via Regulating Arabidopsis EC1 Gene Expression.Plant physiology · 2017Article
- Genome-wide transcriptome analysis of female-sterile rice ovule shed light on its abortive mechanism.Planta · 2016Article
- Cross-Talk Between Sporophyte and Gametophyte Generations Is Promoted by CHD3 Chromatin Remodelers in Arabidopsis thaliana.Genetics · 2016Article
- Depletion of Key Meiotic Genes and Transcriptome-Wide Abiotic Stress Reprogramming Mark Early Preparatory Events Ahead of Apomeiotic Transition.Frontiers in plant science · 2016Article
- Chromatin dynamics during cellular differentiation in the female reproductive lineage of flowering plants.The Plant journal : for cell and molecular biology · 2015Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant lacking an embryo sac in order to identify embryo sac expressed genes in this model organism. The influences of the embryo sac on the surrounding sporophytic tissues were previously thought to be negligible or nonexistent; we investigated the extent of these interactions by transcriptome analysis.
resultsWe identified 1,260 genes as embryo sac expressed by analyzing both our dataset and a recently reported dataset, obtained by a similar approach, using three statistical procedures. Spatial expression of nine genes (for instance a central cell expressed trithorax-like gene, an egg cell expressed gene encoding a kinase, and a synergid expressed gene encoding a permease) validated our approach. We analyzed mutants in five of the newly identified genes that exhibited developmental anomalies during reproductive development. A total of 527 genes were identified for their expression in ovules of mutants lacking an embryo sac, at levels that were twofold higher than in the wild type.
conclusionIdentification of embryo sac expressed genes establishes a basis for the functional dissection of embryo sac development and function. Sporophytic gain of expression in mutants lacking an embryo sac suggests that a substantial portion of the sporophytic transcriptome involved in carpel and ovule development is, unexpectedly, under the indirect influence of the embryo sac.
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