ArticleThe EMBO journal2007
Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- A cyclin-polarity feedback network ensures healthy cell proliferation.Nature communications · 2026Article
- Global genetic rewiring during compensatory evolution in the yeast polarity network.EMBO reports · 2026Article
- Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast.Nature communications · 2023Article
- Amino acid substitutions in specific proteins correlate with farnesol unresponsiveness in Candida albicans.BMC genomics · 2023Article
- Article
- HJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Crystal Structure of the Epo1-Bem3 Complex for Bud Growth.International journal of molecular sciences · 2021Article
- The mitotic exit network regulates the spatiotemporal activity of Cdc42 to maintain cell size.The Journal of cell biology · 2021Article
- Review
- A time-resolved interaction analysis of Bem1 reconstructs the flow of Cdc42 during polar growth.Life science alliance · 2020Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Mechanistic insights into actin-driven polarity site movement in yeast.Molecular biology of the cell · 2020Article
- Cell-cycle control of cell polarity in yeast.The Journal of cell biology · 2019Article
- Regulation of small GTPase activity by G1 cyclins.Small GTPases · 2019Review
- Self-organization principles of intracellular pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Article
- Cell Polarity in Yeast.Annual review of cell and developmental biology · 2017Review
- Evolutionary dynamics in the fungal polarization network, a mechanistic perspective.Biophysical reviews · 2017Review
- Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization.eLife · 2017Article
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Authors and funding
6 authors.
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Abstract
Site-specific activation of the Rho-type GTPase Cdc42p is critical for the establishment of cell polarity. Here we investigated the role and regulation of the GTPase-activating enzymes (GAPs) Bem2p and Bem3p for Cdc42p activation and actin polarization at bud emergence in Saccharomyces cerevisiae. Bem2p and Bem3p are localized throughout the cytoplasm and the cell cortex in unbudded G1 cells, but accumulate at sites of polarization after bud emergence. Inactivation of Bem2p results in hyperactivation of Cdc42p and polarization toward multiple sites. Bem2p and Bem3p are hyperphosphorylated at bud emergence most likely by the Cdc28p-Cln2p kinase. This phosphorylation appears to inhibit their GAP activity in vivo, as non-phosphorylatable Bem3p mutants are hyperactive and interfere with Cdc42p activation. Taken together, our results indicate that Bem2p and Bem3p may function as global inhibitors of Cdc42p activation during G1, and their inactivation by the Cdc28p/Cln kinase contributes to site-specific activation of Cdc42p at bud emergence.
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