ReviewJournal of biosciences2007
Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette.
Review in Journal of biosciences, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Predicting clinical outcome ofMicrobial genomics · 2025Article
- Two novel bacteriophages isolated from the environment that can help control activated sludge foaming.Folia microbiologica · 2024Article
- Metagenomes of rectal swabs in larger, advanced stage cervical cancers have enhanced mucus degrading functionalities and distinct taxonomic structure.BMC cancer · 2022Article
- Functional Dissection of P1 Bacteriophage Holin-like Proteins Reveals the Biological Sense of P1 Lytic System Complexity.International journal of molecular sciences · 2022Article
- VirulentFrontiers in microbiology · 2021Article
- A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets.Journal of animal physiology and animal nutrition · 2020Article
- A Cytoplasmic Antiholin Is Embedded In Frame with the Holin in a Lactobacillus fermentum Bacteriophage.Applied and environmental microbiology · 2018Article
- ydfD encodes a novel lytic protein in Escherichia coli.FEMS microbiology letters · 2016Article
- Holins in bacteria, eukaryotes, and archaea: multifunctional xenologues with potential biotechnological and biomedical applications.Journal of bacteriology · 2015Review
- Genomic investigation of lysogen formation and host lysis systems of the Salmonella temperate bacteriophage SPN9CC.Applied and environmental microbiology · 2014Article
- Complete nucleotide sequence of Bacillus subtilis (natto) bacteriophage PM1, a phage associated with disruption of food production.Virus genes · 2013Article
- Evolutionary potential, cross-stress behavior and the genetic basis of acquired stress resistance in Escherichia coli.Molecular systems biology · 2013Article
- Characterization of DLP12 Prophage Membrane Associated Protein: HolinGFP.Indian journal of microbiology · 2012Article
- The bacteriophage WORiC is the active phage element in wRi of Drosophila simulans and represents a conserved class of WO phages.BMC microbiology · 2011Article
- Role of DLP12 lysis genes in Escherichia coli biofilm formation.Microbiology (Reading, England) · 2011Article
- Controlling biofilm formation, prophage excision and cell death by rewiring global regulator H-NS of Escherichia coli.Microbial biotechnology · 2010Article
- Control and benefits of CP4-57 prophage excision in Escherichia coli biofilms.The ISME journal · 2009Article
- Protein translation and cell death: the role of rare tRNAs in biofilm formation and in activating dormant phage killer genes.PloS one · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage induced lysis of host bacterial cell is mediated by a two component cell lysis cassette comprised of holin and lysozyme. Prophages are integrated forms of bacteriophages in bacterial genomes providing a repertoire for bacterial evolution. Analysis using the prophage database (http://bicmku.in:8082) constructed by us showed 47 prophages were associated with putative two component cell lysis genes. These proteins cluster into four different subgroups. In this process, a putative holin (essd) and endolysin (ybcS), encoded by the defective lambdoid prophage DLP12 was found to be similar to two component cell lysis genes in functional bacteriophages like p21 and P1. The holin essd was found to have a characteristic dual start motif with two transmembrane regions and C-terminal charged residues as in class II holins. Expression of a fusion construct of essd in Escherichia coli showed slow growth. However, under appropriate conditions, this protein could be over expressed and purified for structure function studies. The second component of the cell lysis cassette, ybcS, was found to have an N-terminal SAR (Signal Arrest Release) transmembrane domain. The construct of ybcS has been over expressed in E.coli and the purified protein was functional, exhibiting lytic activity against E.coli and Salmonella typhi cell wall substrate. Such targeted sequence- structure-function characterization of proteins encoded by cryptic prophages will help understand the contribution of prophage proteins to bacterial evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.