ArticleTransgenic research2008
Resistance to Grapevine leafroll associated virus-2 is conferred by post-transcriptional gene silencing in transgenic Nicotiana benthamiana.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus.International journal of genomics · 2016Article
- Delay in virus accumulation and low virus transmission from transgenic rice plants expressing Rice tungro spherical virus RNA.Virus genes · 2012Article
- RNAi-mediated resistance to Cassava brown streak Uganda virus in transgenic cassava.Molecular plant pathology · 2011Article
- RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease.Molecular plant pathology · 2011Article
- Transgenic peppers that are highly tolerant to a new CMV pathotype.Plant cell reports · 2009Article
- Expression of beet yellows virus coat protein cDNA to create transgenic resistance in plants.Doklady. Biochemistry and biophysicsArticle
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grapevine leafroll-associated virus-2 (GLRaV-2) is an important component of the leafroll disease complex in grapevine. We have previously sequenced the GLRaV-2 genome and identified the coat protein (CP) gene. The objective of this study is to test the concept of pathogen-derived resistance against a closterovirus associated with grapevine leafroll disease. Because GLRaV-2 is capable of infecting Nicotiana benthamiana, we decided to test the concept on this herbaceous host. Thirty-seven T(0) transgenic N. benthamiana plants expressing the GLRaV-2 CP gene were regenerated following Agrobacterium-mediated transformation. Disease resistance was evaluated in greenhouse-grown T(1) and T(2) plants by mechanical inoculation with GLRaV-2. Although all the inoculated non-transgenic plants showed symptoms 2-4 weeks post inoculation, various numbers of transgenic plants (16-100%) in 14 of 20 T(1) lines tested were not infected. In these resistant plants, GLRaV-2 was not detectable by enzyme linked immunosorbent assay. Although virus resistance was confirmed in T(2) progenies, the percentage of resistant plants was generally lower (0-63%) than that of the corresponding T(1) lines (0-100%). Northern blot and nuclear run-off results showed that virus resistance in the transgenic plants was consistently associated with the low level of transgene RNA transcript suggesting a post-transcriptional gene silencing. The success of pathogen-derived resistance to GLRaV-2 in transgenic N. benthamiana plants represents the first step towards eventual control of the leafroll disease in grapevines using this strategy.
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