ArticlePloS one2007
Non-invasive in vivo imaging of calcium signaling in mice.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Mitochondrial fusion is frequent in skeletal muscle and supports excitation-contraction coupling.The Journal of cell biology · 2014Trial
- Illuminating ongoing pain in mice with novel bioluminescent imaging.The journal of pain · 2026Article
- PhAT: A Flexible Open-Source GUI-Driven Toolkit for Photometry Analysis.Current protocols · 2023Article
- Lighting Up CaCells · 2021Review
- A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals.Function (Oxford, England) · 2021Article
- RedquorinXS Mutants with Enhanced Calcium Sensitivity and Bioluminescence Output Efficiently Report Cellular and Neuronal Network Activities.International journal of molecular sciences · 2020Article
- Visualization of Mitochondrial CaInternational journal of molecular sciences · 2019Article
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging.ACS chemical biology · 2019Article
- Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae.Bio-protocol · 2017Article
- The reinvention of twentieth century microscopy for three-dimensional imaging.Immunology and cell biology · 2017Review
- Spying on organelle Ca²⁺ in living cells: the mitochondrial point of view.Journal of endocrinological investigation · 2015Review
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Interactions between sarco-endoplasmic reticulum and mitochondria in cardiac and skeletal muscle - pivotal roles in Ca²⁺ and reactive oxygen species signaling.Journal of cell science · 2013Review
- Effect of calcium on the oxidative phosphorylation cascade in skeletal muscle mitochondria.Biochemistry · 2013Article
- Light-emitting channelrhodopsins for combined optogenetic and chemical-genetic control of neurons.PloS one · 2013Article
- Red fluorescent protein-aequorin fusions as improved bioluminescent Ca2+ reporters in single cells and mice.PloS one · 2011Article
- Autofluorescence-free in vivo multicolor imaging using upconversion fluoride nanocrystals.Lasers in medical science · 2010Article
- 'In vivo' optical approaches to angiogenesis imaging.Angiogenesis · 2010Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)]) rises in live animals using bioluminescence imaging (BLI). Transgenic mice conditionally expressing the Ca(2+)-sensitive bioluminescent reporter GFP-aequorin targeted to the mitochondrial matrix were studied in several experimental paradigms. Rapid [Ca(2+)] rises inside the mitochondrial matrix could be readily detected during single-twitch muscle contractions. Whole body patterns of [Ca(2+)] were monitored in freely moving mice and during epileptic seizures. Furthermore, variations in mitochondrial [Ca(2+)] correlated to behavioral components of the sleep/wake cycle were observed during prolonged whole body recordings of newborn mice. This non-invasive imaging technique opens new avenues for the analysis of Ca(2+) signaling whenever whole body information in freely moving animals is desired, in particular during behavioral and developmental studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.