ArticleThe Journal of biological chemistry2007
Functional SmpB-ribosome interactions require tmRNA.
Article in The Journal of biological chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- E. coli metabolic protein aldehyde-alcohol dehydrogenase-E binds to the ribosome: a unique moonlighting action revealed.Scientific reports · 2016Article
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- Resolving nonstop translation complexes is a matter of life or death.Journal of bacteriology · 2014Review
- The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes.Frontiers in microbiology · 2014Review
- Ends of the line for tmRNA-SmpB.Frontiers in microbiology · 2014Article
- Ribosomes regulate the stability and action of the exoribonuclease RNase R.The Journal of biological chemistry · 2013Article
- Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA).The Journal of biological chemistry · 2013Article
- Proteobacterial ArfA peptides are synthesized from non-stop messenger RNAs.The Journal of biological chemistry · 2012Article
- Ribosome purification approaches for studying interactions of regulatory proteins and RNAs with the ribosome.Methods in molecular biology (Clifton, N.J.) · 2012Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.Journal of nucleic acids · 2011Article
- Non-stop mRNA decay initiates at the ribosome.Molecular microbiology · 2010Article
- Role of the C-terminal tail of SmpB in the early stage of trans-translation.RNA (New York, N.Y.) · 2010Article
- Beyond ribosome rescue: tmRNA and co-translational processes.FEBS letters · 2010Review
- Structural features of the tmRNA-ribosome interaction.RNA (New York, N.Y.) · 2009Article
- Kinetics of paused ribosome recycling in Escherichia coli.Journal of molecular biology · 2009Article
- Escherichia coli tmRNA lacking pseudoknot 1 tags truncated proteins in vivo and in vitro.RNA (New York, N.Y.) · 2009Article
- Quality control of bacterial mRNA decoding and decay.Biochimica et biophysica acta · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 3 countries.
Funding
Abstract
Small protein B (SmpB) is a requisite component of the transfer messenger RNA (tmRNA)-mediated bacterial translational quality control system known as trans-translation. The initial binding of tmRNA and its subsequent accommodation into the ribosomal A-site are activities intimately linked to SmpB protein function. From a mechanistic perspective, two key unanswered questions that require further investigation are: 1) what constitutes a stalled ribosome recognition complex and 2) does SmpB pre-bind ribosomes to recruit tmRNA. We have assessed, both in vivo and in vitro, the nature and stability of free SmpB interactions with stalled ribosomes and examined whether these interactions are functionally relevant. We present evidence to demonstrate that interaction of free SmpB with ribosomes is salt sensitive and significantly more labile than interaction of the SmpB.tmRNA complex with ribosomes. Upon dissociation of 70 S ribosomes SmpB partitions primarily with tmRNA rather than ribosomal subunits. This finding is consistent with biochemical and structural data demonstrating that tmRNA is the high-affinity binding partner of SmpB. Moreover, we show that under normal physiological conditions roughly similar numbers of SmpB and tmRNA molecules are present in cells. Our investigations also reveal that upon induction of a nonstop mRNA, SmpB is enriched in stalled ribosome fractions only in the presence of tmRNA. Based on these findings, we conclude that SmpB does not pre-bind stalled ribosome and that functional SmpB-stalled ribosome interactions require tmRNA. We propose that a 1:1:1 complex of SmpB.tmRNA.EF-Tu(GTP) recognizes and binds a stalled ribosome to initiate trans-translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.