ArticleRetrovirology2007
Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains.
Article in Retrovirology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 73 citations in OpenAlex.
- Neutron Reflectometry and Molecular Simulations Demonstrate HIV-1 Nef Homodimer Formation on Model Lipid Bilayers.Journal of molecular biology · 2023Article
- Immunometabolic Reprogramming in Response to HIV Infection Is Not Fully Normalized by Suppressive Antiretroviral Therapy.Viruses · 2022Article
- What do secreted phospholipases ABiochimie · 2021Review
- Selective Disruption of SERINC5 Antagonism by Nef Impairs SIV Replication in Primary CD4Journal of virology · 2021Article
- The autophagy protein ATG9A promotes HIV-1 infectivity.Retrovirology · 2019Article
- Membrane-wrapped nanoparticles probe divergent roles of GM3 and phosphatidylserine in lipid-mediated viral entry pathways.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Associations of gender and serum total cholesterol with CD4+ T cell count and HIV RNA load in antiretroviral-naïve individuals in Addis Ababa.BMC public health · 2018Article
- The host-cell restriction factor SERINC5 restricts HIV-1 infectivity without altering the lipid composition and organization of viral particles.The Journal of biological chemistry · 2017Article
- Article
- SERINC as a Restriction Factor to Inhibit Viral Infectivity and the Interaction with HIV.Journal of immunology research · 2017Review
- Spotlight on HIV-1 Nef: SERINC3 and SERINC5 Identified as Restriction Factors Antagonized by the Pathogenesis Factor.Viruses · 2015Article
- Targeting of the hydrophobic metabolome by pathogens.Traffic (Copenhagen, Denmark) · 2015Review
- Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.Scientific reports · 2015Article
- HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells.Retrovirology · 2014Article
- Target silencing of components of the conserved oligomeric Golgi complex impairs HIV-1 replication.Virus research · 2014Article
- Lentiviral Nef suppresses iron uptake in a strain specific manner through inhibition of Transferrin endocytosis.Retrovirology · 2014Article
- Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages.Frontiers in microbiology · 2014Review
- HIV-1 Nef is transferred from expressing T cells to hepatocytic cells through conduits and enhances HCV replication.PloS one · 2014Article
- The activity of Nef on HIV-1 infectivity.Frontiers in microbiology · 2014Review
- HIV-1 Nef promotes the localization of Gag to the cell membrane and facilitates viral cell-to-cell transfer.Retrovirology · 2013Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Nef protein of Human Immunodeficiency Viruses optimizes viral spread in the infected host by manipulating cellular transport and signal transduction machineries. Nef also boosts the infectivity of HIV particles by an unknown mechanism. Recent studies suggested a correlation between the association of Nef with lipid raft microdomains and its positive effects on virion infectivity. Furthermore, the lipidome analysis of HIV-1 particles revealed a marked enrichment of classical raft lipids and thus identified HIV-1 virions as an example for naturally occurring membrane microdomains. Since Nef modulates the protein composition and function of membrane microdomains we tested here if Nef also has the propensity to alter microdomain lipid composition.
resultsQuantitative mass spectrometric lipidome analysis of highly purified HIV-1 particles revealed that the presence of Nef during virus production from T lymphocytes enforced their raft character via a significant reduction of polyunsaturated phosphatidylcholine species and a specific enrichment of sphingomyelin. In contrast, Nef did not significantly affect virion levels of phosphoglycerolipids or cholesterol. The observed alterations in virion lipid composition were insufficient to mediate Nef's effect on particle infectivity and Nef augmented virion infectivity independently of whether virus entry was targeted to or excluded from membrane microdomains. However, altered lipid compositions similar to those observed in virions were also detected in detergent-resistant membrane preparations of virus producing cells.
conclusionNef alters not only the proteome but also the lipid composition of host cell microdomains. This novel activity represents a previously unrecognized mechanism by which Nef could manipulate HIV-1 target cells to facilitate virus propagation in vivo.
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