ArticleJournal of bacteriology2007
Sequencing the botulinum neurotoxin gene and related genes in Clostridium botulinum type E strains reveals orfx3 and a novel type E neurotoxin subtype.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Guideline
- Botulinum neurotoxins exploit host digestive proteases to boost their oral toxicity via activating OrfXs/P47.Nature structural & molecular biology · 2025Article
- Development of a loop-mediated isothermal amplification method for the rapid detection of Clostridium botulinum serotypes E and F.Molecular biology reports · 2024Article
- Clostridial Neurotoxins: Structure, Function and Implications to Other Bacterial Toxins.Microorganisms · 2021Review
- Pan-Genomic Analysis ofToxins · 2020Article
- Variations in the Botulinum Neurotoxin Binding Domain and the Potential for Novel Therapeutics.Toxins · 2018Review
- A Three Monoclonal Antibody Combination Potently Neutralizes Multiple Botulinum Neurotoxin Serotype E Subtypes.Toxins · 2018Article
- Article
- Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.Applied and environmental microbiology · 2015Article
- An atypical outbreak of food-borne botulism due to Clostridium botulinum types B and E from ham.Journal of clinical microbiology · 2015Article
- Positive regulation of botulinum neurotoxin gene expression by CodY in Clostridium botulinum ATCC 3502.Applied and environmental microbiology · 2014Article
- Two novel toxin variants revealed by whole-genome sequencing of 175 Clostridium botulinum type E strains.Applied and environmental microbiology · 2014Article
- Article
- Plasmid-borne type E neurotoxin gene clusters in Clostridium botulinum strains.Applied and environmental microbiology · 2013Article
- Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum.BMC genomics · 2013Article
- Two-component signal transduction system CBO0787/CBO0786 represses transcription from botulinum neurotoxin promoters in Clostridium botulinum ATCC 3502.PLoS pathogens · 2013Article
- Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes.Applied and environmental microbiology · 2012Article
- Review
- Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype.BMC microbiology · 2012Article
- Analysis of Clostridium botulinum serotype E strains by using multilocus sequence typing, amplified fragment length polymorphism, variable-number tandem-repeat analysis, and botulinum neurotoxin gene sequencing.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three Clostridium botulinum type E strains were sequenced for the botulinum neurotoxin (BoNT) gene cluster, and 11 type E strains, representing a wide biodiversity, were sequenced for the bont/E gene. The total length of the BoNT/E gene cluster was 12,908 bp, and a novel gene (partial) designated orfx3, together with the complete orfx2 gene, was identified in the three type E strains for the first time. Apart from orfx3, the structure and organization of the neurotoxin gene cluster of the three strains were identical to those of previously published ones. Only minor differences (</=3%) in the nucleotide sequences of the gene cluster components were observed among the three strains and the published BoNT/E-producing clostridia. The orfx3, orfx2, orfx1, and p47 gene sequences of the three type E strains shared homologies of 81%, 67 to 76%, 78 to 79%, and 79 to 85%, respectively, with published sequences for type A1 and A2 C. botulinum. Analysis of bont/E from the 14 type E strains and 19 previously published BoNT/E-producing clostridia revealed six neurotoxin subtypes, with a new distinct subtype consisting of three Finnish isolates alone. The amino acid sequence of the subtype E6 neurotoxin differed 3 to 6% from the other subtypes, suggesting that these subtype E6 neurotoxins may possess specific antigenic or functional properties.
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Registered trials
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