ArticleJournal of bacteriology2007
A new O-antigen gene cluster has a key role in the virulence of the Escherichia coli meningitis clone O45:K1:H7.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
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- A convergent evolutionary pathway attenuating cellulose production drives enhanced virulence of some bacteria.Nature communications · 2024Article
- Structural and Genetic Identification of the O-Antigen from anInternational journal of molecular sciences · 2023Article
- O-Polysaccharides of LPS ModulateMicroorganisms · 2023Article
- Genomic characterization of endemic diarrheagenic Escherichia coli and Escherichia albertii from infants with diarrhea in Vietnam.PLoS neglected tropical diseases · 2023Article
- Platelets andBiomedicines · 2022Review
- Investigation of the enzymes required for the biosynthesis of an unusual formylated sugar in the emerging human pathogen Helicobacter canadensis.Protein science : a publication of the Protein Society · 2021Article
- Two EnteropathogenicJournal of microbiology and biotechnology · 2021Article
- Distribution of Novel Og Types in Shiga Toxin-Producing Escherichia coli Isolated from Healthy Cattle.Journal of clinical microbiology · 2021Article
- Structure and genetics of Escherichia coli O antigens.FEMS microbiology reviews · 2020Review
- Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel andJournal of clinical microbiology · 2020Article
- Article
- Genetics and evolution of Yersinia pseudotuberculosis O-specific polysaccharides: a novel pattern of O-antigen diversity.FEMS microbiology reviews · 2017Review
- Enterohemorrhagic Escherichia coli Hybrid Pathotype O80:H2 as a New Therapeutic Challenge.Emerging infectious diseases · 2016Article
- Article
- Rapid and Easy In Silico Serotyping of Escherichia coli Isolates by Use of Whole-Genome Sequencing Data.Journal of clinical microbiology · 2015Article
- Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping.Journal of clinical microbiology · 2015Article
- Bacterial Sugar 3,4-Ketoisomerases: Structural Insight into Product Stereochemistry.Biochemistry · 2015Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new highly pathogenic clone of Escherichia coli meningitis strains harboring the unusual serogroup O45 has recently emerged in France. To gain insight into the pathogenicity of this new clone, we investigated the possible role of antigen O45 in the virulence of strain S88 (O45:K1:H7), representative of this emerging clone. We first showed that the S88 O-antigen gene cluster sequence differs from that of O45 in the reference strain E. coli 96-3285, suggesting that the two O45 polysaccharides, while probably sharing a community of epitopes, represent two different antigens. The unique functional organization of the two O-antigen gene clusters and the low DNA sequence homology of the orthologous genes suggest that the two loci originated from a common ancestor and have since undergone multiple recombination events. Phylogenetic analysis based on the flanking gene gnd sequences indicates that the S88 antigen O45 (O45(S88)) gene cluster may have been acquired, at least in part, from another member of the Enterobacteriaceae. Mutagenesis of the O45(S88) antigen gene cluster was used for functional analysis of the loci and revealed the crucial role of the O polysaccharide in S88 virulence in a neonatal rat meningitis model. We also developed a PCR method to specifically identify the O45(S88) antigen gene cluster. Together, our findings suggest that horizontal acquisition of a new O-antigen gene cluster, at least partly from another species, may have been a key event in the emergence and virulence of the E. coli O45:K1:H7 clone in France.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.