ArticleApplied and environmental microbiology2007
Analysis of the mycosamine biosynthesis and attachment genes in the nystatin biosynthetic gene cluster of Streptomyces noursei ATCC 11455.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 39 citations in OpenAlex.
- EneA of Aspergillus fumigatus is a regulator of secondary metabolism and enhances nscA expression in presence of polyenes and Streptomyces.Scientific reports · 2026Article
- Research and Application of the Polyene Macrolide Antibiotic Nystatin.Molecules (Basel, Switzerland) · 2026Review
- Isolation of Streptomyces inhibiting multiple-phytopathogenic fungi and characterization of lucensomycin biosynthetic gene cluster.Scientific reports · 2024Article
- Effect of Post-Polyketide Synthase Modification Groups on Property and Activity of Polyene Macrolides.Antibiotics (Basel, Switzerland) · 2023Review
- New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining.Antibiotics (Basel, Switzerland) · 2022Review
- A Machine Learning Bioinformatics Method to Predict Biological Activity from Biosynthetic Gene Clusters.Journal of chemical information and modeling · 2021Article
- A Hierarchical Network of Four Regulatory Genes Controlling Production of the Polyene Antibiotic Candicidin inApplied and environmental microbiology · 2020Article
- Stimulated Biosynthesis of an C10-Deoxy Heptaene NPP B2Frontiers in microbiology · 2020Article
- Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids byFrontiers in microbiology · 2018Article
- Improved recovery and biological activities of an engineered polyene NPP analogue in Pseudonocardia autotrophica.Journal of industrial microbiology & biotechnology · 2017Article
- Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation.Applied microbiology and biotechnology · 2016Review
- In vitro characterization of LmbK and LmbO: identification of GDP-D-erythro-α-D-gluco-octose as a key intermediate in lincomycin A biosynthesis.Journal of the American Chemical Society · 2014Article
- Biosynthesis and pathway engineering of antifungal polyene macrolides in actinomycetes.Journal of industrial microbiology & biotechnology · 2013Review
- New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei.Applied and environmental microbiology · 2011Article
- The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.Chembiochem : a European journal of chemical biology · 2008Article
- Increasing carbohydrate diversity via amine oxidation: aminosugar, hydroxyaminosugar, nitrososugar, and nitrosugar biosynthesis in bacteria.Current opinion in chemical biology · 2008Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polyene macrolide antibiotic nystatin produced by Streptomyces noursei contains a deoxyaminosugar mycosamine moiety attached to the C-19 carbon of the macrolactone ring through the beta-glycosidic bond. The nystatin biosynthetic gene cluster contains three genes, nysDI, nysDII, and nysDIII, encoding enzymes with presumed roles in mycosamine biosynthesis and attachment as glycosyltransferase, aminotransferase, and GDP-mannose dehydratase, respectively. In the present study, the functions of these three genes were analyzed. The recombinant NysDIII protein was expressed in Escherichia coli and purified, and its in vitro GDP-mannose dehydratase activity was demonstrated. The nysDI and nysDII genes were inactivated individually in S. noursei, and analyses of the resulting mutants showed that both genes produced nystatinolide and 10-deoxynystatinolide as major products. Expression of the nysDI and nysDII genes in trans in the respective mutants partially restored nystatin biosynthesis in both cases, supporting the predicted roles of these two genes in mycosamine biosynthesis and attachment. Both antifungal and hemolytic activities of the purified nystatinolides were shown to be strongly reduced compared to those of nystatin, confirming the importance of the mycosamine moiety for the biological activity of nystatin.
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