ArticlePlant physiology2007
The ubiquitin-specific protease subfamily UBP3/UBP4 is essential for pollen development and transmission in Arabidopsis.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 82 citations in OpenAlex.
- Genome-wide characterization of DUB genes in Betula platyphylla and BpUBP3 function in pollen tube growth.Plant molecular biology · 2026Article
- The Ubiquitin-Proteasome System in Flowering Plant Reproduction: Mechanisms, Functional Diversity, and Regulatory Networks.Plants (Basel, Switzerland) · 2026Review
- Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening.Nature communications · 2026Article
- Genomic and Effector-Based Insights Into Austropuccinia psidii-Host Interactions Informing RNAi and Resistance Development.Molecular plant pathology · 2026Review
- Upregulation of deubiquitinase UBP16 induced by rice stripe virus infection stabilizes SHMT1 to suppress ROS accumulation and facilitate virus infection in Nicotiana benthamiana.Stress biology · 2025Article
- Genome-Wide Identification and Comprehensive Analysis of Ubiquitin-Specific Protease Gene Family in Soybean (International journal of molecular sciences · 2025Article
- Ubiquitin-specific protease UBP14 stabilizes HY5 by deubiquitination to promote photomorphogenesis inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genome-wide identification and expression analysis of Ubiquitin-specific protease gene family in maize (Zea mays L.).BMC plant biology · 2024Article
- Transcriptome-wide association mapping provides insights into the genetic basis and candidate genes governing flowering, maturity and seed weight in rice bean (Vigna umbellata).BMC plant biology · 2024Article
- An insight into the roles of ubiquitin-specific proteases in plants: development and growth, morphogenesis, and stress response.Frontiers in plant science · 2024Review
- Plant deubiquitinases: from structure and activity to biological functions.Plant cell reports · 2023Review
- The deubiquitinases UBP12 and UBP13 integrate with the E3 ubiquitin ligase XBAT35.2 to modulate VPS23A stability in ABA signaling.Science advances · 2022Article
- HsfA1a confers pollen thermotolerance through upregulating antioxidant capacity, protein repair, and degradation inHorticulture research · 2022Article
- Ubiquitin-specific proteases UBP12 and UBP13 promote shade avoidance response by enhancing PIF7 stability.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Plant protease as regulator and signaling molecule for enhancing environmental stress-tolerance.Plant cell reports · 2021Review
- The DUB family in Populus: identification, characterization, evolution and expression patterns.BMC genomics · 2021Article
- OsLMP1, Encoding a Deubiquitinase, Regulates the Immune Response in Rice.Frontiers in plant science · 2021Article
- Ubiquitination in Plant Meiosis: Recent Advances and High Throughput Methods.Frontiers in plant science · 2021Review
- Ubiquitin carboxyl-terminal hydrolases are required for period maintenance of the circadian clock at high temperature in Arabidopsis.Scientific reports · 2019Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deubiquitinating enzymes are essential to the ubiquitin (Ub)/26S proteasome system where they release Ub monomers from the primary translation products of poly-Ub and Ub extension genes, recycle Ubs from polyubiquitinated proteins, and reverse the effects of ubiquitination by releasing bound Ubs from individual targets. The Ub-specific proteases (UBPs) are one large family of deubiquitinating enzymes that bear signature cysteine and histidine motifs. Here, we genetically characterize a UBP subfamily in Arabidopsis (Arabidopsis thaliana) encoded by paralogous UBP3 and UBP4 genes. Whereas homozygous ubp3 and ubp4 single mutants do not display obvious phenotypic abnormalities, double-homozygous mutant individuals could not be created due to a defect in pollen development and/or transmission. This pollen defect was rescued with a transgene encoding wild-type UBP3 or UBP4, but not with a transgene encoding an active-site mutant of UBP3, indicating that deubiquitination activity of UBP3/UBP4 is required. Nuclear DNA staining revealed that ubp3 ubp4 pollen often fail to undergo mitosis II, which generates the two sperm cells needed for double fertilization. Substantial changes in vacuolar morphology were also evident in mutant grains at the time of pollen dehiscence, suggesting defects in vacuole and endomembrane organization. Even though some ubp3 ubp4 pollen could germinate in vitro, they failed to fertilize wild-type ovules even in the absence of competing wild-type pollen. These studies provide additional evidence that the Ub/26S proteasome system is important for male gametogenesis in plants and suggest that deubiquitination of one or more targets by UBP3/UBP4 is critical for the development of functional pollen.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.