ArticleBiophysical journal2008
Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 85 citations in OpenAlex.
- Dressed Up to the Nines: The Interplay of Phytohormones Signaling and Redox Metabolism During Plant Response to Drought.Plants (Basel, Switzerland) · 2025Review
- The cell surface is the place to be for brassinosteroid perception and responses.Nature plants · 2024Review
- Exploring the Binding Affinity of the ARR2 GARP DNA Binding Domain via Comparative Methods.Genes · 2023Article
- Protein-Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Review
- The Promoter Analysis ofPlants (Basel, Switzerland) · 2022Article
- Determination of G-protein-coupled receptor oligomerization by molecular brightness analyses in single cells.Nature protocols · 2021Article
- State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks.International journal of molecular sciences · 2020Review
- Regulation of Three Key Kinases of Brassinosteroid Signaling Pathway.International journal of molecular sciences · 2020Review
- Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.Biophysical reviews · 2018Review
- Two SERK Receptor-Like Kinases Interact with EMS1 to Control Anther Cell Fate Determination.Plant physiology · 2017Article
- Structural insights into the negative regulation of BRI1 signaling by BRI1-interacting protein BKI1.Cell research · 2014Article
- Nod factor receptors form heteromeric complexes and are essential for intracellular infection in medicago nodules.The Plant cell · 2014Article
- Crystal structures of the phosphorylated BRI1 kinase domain and implications for brassinosteroid signal initiation.The Plant journal : for cell and molecular biology · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
- Review
- FRET-FLIM applications in plant systems.Protoplasma · 2014Review
- A simple in vitro method to measure autophosphorylation of protein kinases.Plant methods · 2013Article
- The brassinosteroid signaling pathway-new key players and interconnections with other signaling networks crucial for plant development and stress tolerance.International journal of molecular sciences · 2013Review
- Simultaneous diffusion and brightness measurements and brightness profile visualization from single fluorescence fluctuation traces of GFP in living cells.European biophysics journal : EBJ · 2012Article
- Constitutive activation of brassinosteroid signaling in the Arabidopsis elongated-D/bak1 mutant.Plant molecular biology · 2012Article
Corrections and comments
- Expression of concern
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Receptor kinases play a key role in the cellular perception of signals. To verify models for receptor activation through dimerization, an experimental system is required to determine the precise oligomerization status of proteins within living cells. Here we show that photon counting histogram analysis and dual-color fluorescence cross correlation spectroscopy are able to monitor fluorescently labeled proteins at the single-molecule detection level in living plant cells. In-frame fusion proteins of the brassinosteroid insensitive 1 (BRI1) receptor and the Arabidopsis thaliana somatic embryogenesis receptor-like kinases 1 and 3 (AtSERK1 and 3) to the enhanced cyan or yellow fluorescent protein were transiently expressed in plant cells. Although no oligomeric structures were detected for AtSERK3, 15% (AtSERK1) to 20% (BRI1) of the labeled proteins in the plasma membrane was found to be present as homodimers, whereas no evidence was found for higher oligomeric complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.