ArticleNucleic acids research2007
An RNA toolbox for single-molecule force spectroscopy studies.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
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- Overstretching Double-Stranded RNA, Double-Stranded DNA, and RNA-DNA Duplexes.Biophysical journal · 2019Article
- A mechanistic study of helicases with magnetic traps.Protein science : a publication of the Protein Society · 2017Review
- Article
- Proximal disruptor aided ligation (ProDAL) of kilobase-long RNAs.RNA biology · 2016Article
- Nanomanipulation of single RNA molecules by optical tweezers.Journal of visualized experiments : JoVE · 2014Article
- Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription.Journal of nanobiotechnology · 2013Article
- A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence.RNA biology · 2013Article
- Helicase-mediated changes in RNA structure at the single-molecule level.RNA biology · 2013Review
- Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry.Nucleic acids research · 2012Article
- Molecular characterization of a novel mycovirus in the cultivated mushroom, Lentinula edodes.Virology journal · 2012Article
- Quantitative analysis of single-molecule RNA-protein interaction.Biophysical journal · 2009Article
- Reinitiated viral RNA-dependent RNA polymerase resumes replication at a reduced rate.Nucleic acids research · 2008Article
- End-joining long nucleic acid polymers.Nucleic acids research · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise, controllable single-molecule force spectroscopy studies of RNA and RNA-dependent processes have recently shed new light on the dynamics and pathways of RNA folding and RNA-enzyme interactions. A crucial component of this research is the design and assembly of an appropriate RNA construct. Such a construct is typically subject to several criteria. First, single-molecule force spectroscopy techniques often require an RNA construct that is longer than the RNA molecules used for bulk biochemical studies. Next, the incorporation of modified nucleotides into the RNA construct is required for its surface immobilization. In addition, RNA constructs for single-molecule studies are commonly assembled from different single-stranded RNA molecules, demanding good control of hybridization or ligation. Finally, precautions to prevent RNase- and divalent cation-dependent RNA digestion must be taken. The rather limited selection of molecular biology tools adapted to the manipulation of RNA molecules, as well as the sensitivity of RNA to degradation, make RNA construct preparation a challenging task. We briefly illustrate the types of single-molecule force spectroscopy experiments that can be performed on RNA, and then present an overview of the toolkit of molecular biology techniques at one's disposal for the assembly of such RNA constructs. Within this context, we evaluate the molecular biology protocols in terms of their effectiveness in producing long and stable RNA constructs.
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