ArticleGenome biology2007
A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- The Biological Roles of microRNAs inInsects · 2024Review
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins inG3 (Bethesda, Md.) · 2020Article
- Activation of Smoothened in the Hedgehog pathway unexpectedly increases GαThe Journal of biological chemistry · 2018Article
- RNA Interference (RNAi) Screening inGenetics · 2018Article
- Multiplex shRNA Screening of Germ Cell Development by in Vivo Transfection of Mouse Testis.G3 (Bethesda, Md.) · 2017Article
- WNK Kinases in Development and Disease.Current topics in developmental biology · 2017Review
- Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells.G3 (Bethesda, Md.) · 2015Article
- Integrative approaches for predicting protein function and prioritizing genes for complex phenotypes using protein interaction networks.Briefings in bioinformatics · 2014Article
- RNAi screening in Drosophila cells and in vivo.Methods (San Diego, Calif.) · 2014Review
- The miR-310/13 cluster antagonizes β-catenin function in the regulation of germ and somatic cell differentiation in the Drosophila testis.Development (Cambridge, England) · 2013Article
- A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells.Genome research · 2013Article
- FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update.Nucleic acids research · 2012Article
- Sequential phosphorylation of smoothened transduces graded hedgehog signaling.Science signaling · 2011Article
- Drosophila RNAi screening in a postgenomic world.Briefings in functional genomics · 2011Review
- A protein network-guided screen for cell cycle regulators in Drosophila.BMC systems biology · 2011Article
- False negative rates in Drosophila cell-based RNAi screens: a case study.BMC genomics · 2011Article
- Reverse genetics in eukaryotes.Biology of the cell · 2010Review
- Automated microscopy for high-content RNAi screening.The Journal of cell biology · 2010Review
- Insights to transcriptional networks by using high throughput RNAi strategies.Briefings in functional genomics · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.