ArticleRNA (New York, N.Y.)2007
Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Unraveling the Role of RNA-Binding Proteins, with a Focus on RPS5, in the Malignant Progression of Hepatocellular Carcinoma.International journal of molecular sciences · 2024Article
- Identification of genomic biomarkers and their pathway crosstalks for deciphering mechanistic links in glioblastoma.IET systems biology · 2023Article
- Eukaryotic Ribosomal Protein S5 of the 40S Subunit: Structure and Function.International journal of molecular sciences · 2023Review
- Evolution of ribosomal protein network architectures.Scientific reports · 2021Article
- Article
- Review
- Role of the uS9/yS16 C-terminal tail in translation initiation and elongation in Saccharomyces cerevisiae.Nucleic acids research · 2019Article
- Ribosomal protein uS7/Rps5 serine-223 in protein kinase-mediated phosphorylation and ribosomal small subunit maturation.Scientific reports · 2018Article
- The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits.Nucleic acids research · 2016Article
- Differential Stoichiometry among Core Ribosomal Proteins.Cell reports · 2015Article
- A dynamic RNA loop in an IRES affects multiple steps of elongation factor-mediated translation initiation.eLife · 2015Article
- Rps5-Rps16 communication is essential for efficient translation initiation in yeast S. cerevisiae.Nucleic acids research · 2014Article
- HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation.Nature structural & molecular biology · 2013Article
- Tricks an IRES uses to enslave ribosomes.Trends in microbiology · 2012Review
- Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Yeast strains with N-terminally truncated ribosomal protein S5: implications for the evolution, structure and function of the Rps5/Rps7 proteins.Nucleic acids research · 2010Article
- Mechanistic role of structurally dynamic regions in Dicistroviridae IGR IRESs.Journal of molecular biology · 2010Article
- Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation.Nucleic acids research · 2009Article
- Identification of copper-responsive genes in an early life stage of the fathead minnow Pimephales promelas.Ecotoxicology (London, England) · 2009Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Ribosomal protein (rp) S5 belongs to a family of ribosomal proteins that includes bacterial rpS7. rpS5 forms part of the exit (E) site on the 40S ribosomal subunit and is essential for yeast viability. Human rpS5 is 67% identical and 79% similar to Saccharomyces cerevisiae rpS5 but lacks a negatively charged (pI approximately 3.27) 21 amino acid long N-terminal extension that is present in fungi. Here we report that replacement of yeast rpS5 with its human homolog yielded a viable yeast strain with a 20%-25% decrease in growth rate. This replacement also resulted in a moderate increase in the heavy polyribosomal components in the mutant strain, suggesting either translation elongation or termination defects, and in a reduction in the polyribosomal association of the elongation factors eEF3 and eEF1A. In addition, the mutant strain was characterized by moderate increases in +1 and -1 programmed frameshifting and hyperaccurate recognition of the UAA stop codon. The activities of the cricket paralysis virus (CrPV) IRES and two mammalian cellular IRESs (CAT-1 and SNAT-2) were also increased in the mutant strain. Consistently, the rpS5 replacement led to enhanced direct interaction between the CrPV IRES and the mutant yeast ribosomes. Taken together, these data indicate that rpS5 plays an important role in maintaining the accuracy of translation in eukaryotes and suggest that the negatively charged N-terminal extension of yeast rpS5 might affect the ribosomal recruitment of specific mRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.