ArticleMolecular endocrinology (Baltimore, Md.)2008
Phosphorylation of steroidogenic factor 1 is mediated by cyclin-dependent kinase 7.
Article in Molecular endocrinology (Baltimore, Md.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Post-translational modification of proteins in the human testis development pathway.Human reproduction update · 2026Review
- Adrenocortical stem cells in health and disease.Nature reviews. Endocrinology · 2025Review
- Emerging roles of cyclin-dependent kinase 7 in health and diseases.Trends in molecular medicine · 2025Review
- Steroidogenic Factor-1 form and function: From phospholipids to physiology.Advances in biological regulation · 2024Review
- Steroidogenic Factor 1, a Goldilocks Transcription Factor from Adrenocortical Organogenesis to Malignancy.International journal of molecular sciences · 2023Review
- Inhibition of NR5A1 Phosphorylation Alleviates a Transcriptional Suppression Defect Caused by a NovelJournal of the Endocrine Society · 2022Article
- A novel variant of NR5A1, p.R350W implicates potential interactions with unknown co-factors or ligands.Frontiers in endocrinology · 2022Article
- Coordination of Multiple Cellular Processes by NR5A1/Nr5a1.Endocrinology and metabolism (Seoul, Korea) · 2020Review
- Steroidogenic Factor 1 (NR5A1) Activates ATF3 Transcriptional Activity.International journal of molecular sciences · 2020Article
- Nutritional conditions regulate transcriptional activity of SF-1 by controlling sumoylation and ubiquitination.Scientific reports · 2016Article
- Hedgehog signaling and steroidogenesis.Annual review of physiology · 2015Review
- Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells.Biochimica et biophysica acta · 2014Article
- cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1.Journal of lipid research · 2013Article
- Adrenocortical stem and progenitor cells: unifying model of two proposed origins.Molecular and cellular endocrinology · 2011Review
- Synergistic activation of the Mc2r promoter by FOXL2 and NR5A1 in mice.Biology of reproduction · 2010Article
- Minireview: steroidogenic factor 1: its roles in differentiation, development, and disease.Molecular endocrinology (Baltimore, Md.) · 2010Review
- Pin1 facilitates the phosphorylation-dependent ubiquitination of SF-1 to regulate gonadotropin beta-subunit gene transcription.Molecular and cellular biology · 2010Article
- Transcriptional Suppression by Transient Recruitment of ARIP4 to Sumoylated nuclear receptor Ad4BP/SF-1.Molecular biology of the cell · 2009Article
- In search of adrenocortical stem and progenitor cells.Endocrine reviews · 2009Review
- Complex assembly on the human CYP17 promoter.Molecular and cellular endocrinology · 2009Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear receptor steroidogenic factor-1 (SF1) is critical for development and function of steroidogenic tissues. Posttranslational modifications are known to influence the transcriptional capacity of SF1, and it was previously demonstrated that serine 203 is phosphorylated. In this paper we report that serine 203 is phosphorylated by a cyclin-dependent kinase 7 (CDK7)-mediated process. As part of the CDK-activating kinase complex, CDK7 is a component of the basal transcription factor TFIIH, and phosphorylation of SF1 as well as SF1-dependent transcription was clearly reduced in cells carrying a mutation that renders the CDK-activating kinase complex unable to interact with the TFIIH core. Coimmunoprecipitation analyses revealed that SF1 and CDK7 reside in the same complex, and kinase assays demonstrated that immunoprecipitated CDK7 and purified TFIIH phosphorylate SF1 in vitro. The CDK inhibitor roscovitine blocked phosphorylation of SF1, and an inactive form of CDK7 repressed the phosphorylation level and the transactivation capacity of SF1. Structural studies have identified phosphoinositides as potential ligands for SF1. Interestingly, we found that mutations designed to block phospholipid binding dramatically decreased the level of SF1 phosphorylation. Together our results suggest a connection between ligand occupation and phosphorylation and association with the basic transcriptional machinery, indicating an intricate regulation of SF1 transactivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.