ArticleBMC developmental biology2007
Ubiquitous expression of the rtTA2S-M2 inducible system in transgenic mice driven by the human hnRNPA2B1/CBX3 CpG island.
Article in BMC developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- Brain-wide distribution of reporter expression in five transgenic tetracycline-transactivator mouse lines.Scientific data · 2019Article
- Divergent Innate and Epithelial Functions of the RNA-Binding Protein HuR in Intestinal Inflammation.Frontiers in immunology · 2018Article
- ERCC1-XPF cooperates with CTCF and cohesin to facilitate the developmental silencing of imprinted genes.Nature cell biology · 2017Article
- The dual role of LSD1 and HDAC3 in STAT5-dependent transcription is determined by protein interactions, binding affinities, motifs and genomic positions.Nucleic acids research · 2017Article
- The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin.The Journal of biological chemistry · 2014Article
- Induced expression of expanded CGG RNA causes mitochondrial dysfunction in vivo.Cell cycle (Georgetown, Tex.) · 2014Article
- Doxycycline-induced expression of transgenic human tumor necrosis factor α in adult mice results in psoriasis-like arthritis.Arthritis and rheumatism · 2013Article
- Identification of a STAT5 target gene, Dpf3, provides novel insights in chronic lymphocytic leukemia.PloS one · 2013Article
- Generation and characterization of an inducible transgenic model for studying mouse esophageal biology.BMC developmental biology · 2012Article
- Identification of quiescent, stem-like cells in the distal female reproductive tract.PloS one · 2012Article
- Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression.PloS one · 2011Article
- Tetracycline-controlled transgene activation using the ROSA26-iM2-GFP knock-in mouse strain permits GFP monitoring of DOX-regulated transgene-expression.BMC developmental biology · 2010Article
- Temporally and spatially controllable gene expression and knockout in mouse urothelium.American journal of physiology. Renal physiology · 2010Article
- HP1gamma function is required for male germ cell survival and spermatogenesis.Epigenetics & chromatin · 2010Article
- A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase.Genesis (New York, N.Y. : 2000) · 2009Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA sensitive, ubiquitously expressed tetracycline inducible system would be a valuable tool in mouse transgenesis. However, this has been difficult to obtain due to position effects observed at different chromosomal sites of transgene integration, which negatively affect expression in many tissues. The aim of this study was to test the utility of a mammalian methylation-free CpG island to drive ubiquitous expression of the sensitive doxycycline (Dox) inducible rtTA2S-M2 Tet-transactivator in transgenic mice.
resultsAn 8 kb genomic fragment from the methylation-free CpG island of the human hnRNPA2B1-CBX3 housekeeping gene locus was tested. In a number of transgenic mouse lines obtained, rtTA2S-M2 expression was detected in many tissues examined. Characterisation of the highest expressing rtTA2S-M2 transgenic mouse line demonstrated Dox-inducible GFP transgene expression in many tissues. Using this line we also show highly sensitive quantitative induction with low doses of Dox of an assayable plasma protein transgene under the control of a Tet Responsive Element (TRE). The utility of this rtTA2S-M2 line for inducible expression in mouse embryos was also demonstrated using a GATA-6 Tet-inducible transgene to show specific phenotypes in the embryonic lung, as well as broader effects resulting from the inducible widespread overexpression of the transgene.
conclusionThe ubiquitously expressing rtTA2S-M2 transgenic mouse line described here provides a very useful tool for studying the effects of the widespread, inducible overexpression of genes during embryonic development and in adult mice.
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