ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
Critical roles for the M3-S2 transduction linker domain in kainate receptor assembly and postassembly trafficking.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Neto2 Phosphorylation Promotes Neuronal Loss in KA-Induced Epileptic Rat Hippocampus by Upregulating the Interaction with GluK2.Neurochemical research · 2026Article
- Clustered mutations in the GRIK2 kainate receptor subunit gene underlie diverse neurodevelopmental disorders.American journal of human genetics · 2021Article
- N-glycan content modulates kainate receptor functional properties.The Journal of physiology · 2017Article
- Auxiliary subunits highlight a role for the LBD-TMD linkers in glutamate receptor desensitization.The Journal of physiology · 2015Article
- Identification of critical functional determinants of kainate receptor modulation by auxiliary protein Neto2.The Journal of physiology · 2015Article
- Trafficking of kainate receptors.Membranes · 2014Review
- Modulation of ionotropic glutamate receptor function by vertebrate galectins.The Journal of physiology · 2014Article
- Agonist binding to the GluK5 subunit is sufficient for functional surface expression of heteromeric GluK2/GluK5 kainate receptors.Cellular and molecular neurobiology · 2013Article
- Kainate receptor post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis.The Journal of biological chemistry · 2013Article
- Dancing partners at the synapse: auxiliary subunits that shape kainate receptor function.Nature reviews. Neuroscience · 2012Review
- Key amino acid residues within the third membrane domains of NR1 and NR2 subunits contribute to the regulation of the surface delivery of N-methyl-D-aspartate receptors.The Journal of biological chemistry · 2012Article
- Synaptic targeting and functional modulation of GluK1 kainate receptors by the auxiliary neuropilin and tolloid-like (NETO) proteins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Channel-opening kinetic mechanism for human wild-type GluK2 and the M867I mutant kainate receptor.Biochemistry · 2010Article
- A series of structurally novel heterotricyclic alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor-selective antagonists.British journal of pharmacology · 2010Article
- Ion-dependent gating of kainate receptors.The Journal of physiology · 2010Review
- Glutamate binding and conformational flexibility of ligand-binding domains are critical early determinants of efficient kainate receptor biogenesis.The Journal of biological chemistry · 2009Article
- Gating motions underlie AMPA receptor secretion from the endoplasmic reticulum.The EMBO journal · 2008Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Kainate receptors (KARs) are neuronal proteins that exhibit a highly polarized distribution in the mammalian CNS. Assembly, intracellular trafficking, and synaptic targeting of KARs and other ionotropic glutamate receptors are processes controlled, in part, by various determinants within the constituent subunit proteins themselves. Here, we demonstrate that the linker region between the M3 and S2 domains, which in current structural models is thought to transduce ligand-binding energy into channel opening, additionally has an essential role in receptor biogenesis. Our results show that this gating-associated domain is engaged at two distinct critical stages of KAR biogenesis: first, during the transition from dimeric to tetrameric assembly states and, second, at a postassembly trafficking checkpoint within the endoplasmic reticulum. Alteration of a basic residue, arginine 663, altered the desensitization properties of the GluR6 kainate receptor in response to glutamate application, and these changes were weakly correlated with intracellular retention of the mutant receptors. Elimination of the positive charge also significantly attenuated oligomerization and stability of the intracellular subunit protein. Furthermore, charge swapping with an adjacent residue, glutamate 662, normalized the receptor physiological behavior and reversed the deficits in assembly and degradation, but only partially restored plasma membrane expression of the receptors. These results reveal a new role for this linker domain in glutamate receptor biogenesis and contribute to understanding the cellular controls of receptor assembly and trafficking, which will be important for relating receptor stoichiometry to their neuronal targeting and function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.