ArticleMolecular biology of the cell2007
Scd5p mediates phosphoregulation of actin and endocytosis by the type 1 phosphatase Glc7p in yeast.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.The Journal of biological chemistry · 2021Article
- Clathrin-mediated endocytosis in budding yeast at a glance.Journal of cell science · 2016Review
- Article
- Phosphorylation Regulates the Endocytic Function of the Yeast Dynamin-Related Protein Vps1.Molecular and cellular biology · 2015Article
- Pan1 regulates transitions between stages of clathrin-mediated endocytosis.Molecular biology of the cell · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis.Molecular systems biology · 2014Article
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
- Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.Journal of cell science · 2012Article
- Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins.Proteomics · 2012Article
- Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth.Molecular biology of the cell · 2012Article
- Lessons from yeast for clathrin-mediated endocytosis.Nature cell biology · 2011Review
- Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins.PLoS biology · 2009Article
- Protein phosphatase type 1-interacting protein Ysw1 is involved in proper septin organization and prospore membrane formation during sporulation.Eukaryotic cell · 2009Article
- Genetic interaction network of the Saccharomyces cerevisiae type 1 phosphatase Glc7.BMC genomics · 2008Article
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5 authors.
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Abstract
Pan1p plays essential roles in both actin and endocytosis in yeast. It interacts with, and regulates the function of, multiple endocytic proteins and actin assembly machinery. Phosphorylation of Pan1p by the kinase Prk1p down-regulates its activity, resulting in disassembly of the endocytic vesicle coat complex and termination of vesicle-associated actin polymerization. In this study, we focus on the mechanism that acts to release Pan1p from phosphorylation inhibition. We show that Pan1p is dephosphorylated by the phosphatase Glc7p, and the dephosphorylation is dependent on the Glc7p-targeting protein Scd5p, which itself is a phosphorylation target of Prk1p. Scd5p links Glc7p to Pan1p in two ways: directly by interacting with Pan1p and indirectly by interacting with the Pan1p-binding protein End3p. Depletion of Glc7p from the cells causes defects in cell growth, actin organization, and endocytosis, all of which can be partially suppressed by deletion of the PRK1 gene. These results suggest that Glc7p antagonizes the activity of the Prk1p kinase in regulating the functions of Pan1p and possibly other actin- and endocytosis-related proteins.
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