ArticleJournal of virology2007
Virp1 is a host protein with a major role in Potato spindle tuber viroid infection in Nicotiana plants.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 96 citations in OpenAlex.
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Viroid Replication, Movement, and the Host Factors Involved.Microorganisms · 2024Review
- Cellular roadmaps of viroid infection.Trends in microbiology · 2023Review
- Spotlight on Plant Bromodomain Proteins.Biology · 2023Review
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- PSTVd infection inFrontiers in plant science · 2023Article
- SilencingPotato research · 2023Article
- A nuclear import pathway exploited by pathogenic noncoding RNAs.The Plant cell · 2022Article
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- Conserved Motifs and Domains in Members ofCells · 2022Article
- Review
- Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).Biomolecules · 2021Article
- Long Noncoding RNAs in Plant Viroids and Viruses: A Review.Pathogens (Basel, Switzerland) · 2020Review
- Article
- Whole-Genome Deep Sequencing Reveals Host-DrivenInternational journal of molecular sciences · 2020Article
- Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants.International journal of molecular sciences · 2020Article
- Article
- A three-dimensional RNA motif mediates directional trafficking of Potato spindle tuber viroid from epidermal to palisade mesophyll cells in Nicotiana benthamiana.PLoS pathogens · 2019Article
- Citrus exocortis viroid causes ribosomal stress in tomato plants.Nucleic acids research · 2019Article
- Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research.Viruses · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viroids are small, circular, single-stranded RNA molecules that, while not coding for any protein, cause several plant diseases. Viroids rely for their infectious cycle on host proteins, most of which are likely to be involved in endogenous RNA-mediated phenomena. Therefore, characterization of host factors interacting with the viroid may contribute to the elucidation of RNA-related pathways of the hosts. Potato spindle tuber viroid (PSTVd) infects several members of the Solanaceae family. In an RNA ligand screening we have previously isolated the tomato protein Virp1 by its ability to specifically interact with PSTVd positive-strand RNA. Virp1 is a bromodomain-containing protein with an atypical RNA binding domain and a nuclear localization signal. Here we investigate the role of Virp1 in the viroid infection cycle by the use of transgenic lines of Nicotiana tabacum and Nicotiana benthamiana that either overexpress the tomato Virp1 RNA or suppress the orthologous Nicotiana genes through RNA silencing. Plants of the Virp1-suppressed lines were not infected by PSTVd or Citrus exocortis viroid through mechanical inoculation, indicating a major role of Virp1 in viroid infection. On the other hand, overexpression of tomato Virp1 in N. tabacum and N. benthamiana plants did not affect PSTVd KF 440-2 infectivity or symptomatology in these species. Transfection experiments with isolated protoplasts revealed that Virp1-suppressed cells were unable to sustain viroid replication, suggesting that resistance to viroid infection in Virp1-suppressed plants is likely the result of cell-autonomous events.
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