ArticleBMC biotechnology2007
pHUSH: a single vector system for conditional gene expression.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Quantitative microscopy reveals dynamics and fate of clustered IRE1α.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Acetylated histone variant H2A.Z is involved in the activation of neo-enhancers in prostate cancer.Nature communications · 2017Article
- A mutation in the viral sensor 2'-5'-oligoadenylate synthetase 2 causes failure of lactation.PLoS genetics · 2017Article
- Article
- Role of the E3 ubiquitin ligase RNF157 as a novel downstream effector linking PI3K and MAPK signaling pathways to the cell cycle.The Journal of biological chemistry · 2017Article
- Metabolic plasticity underpins innate and acquired resistance to LDHA inhibition.Nature chemical biology · 2016Article
- CCAT1 is an enhancer-templated RNA that predicts BET sensitivity in colorectal cancer.The Journal of clinical investigation · 2016Article
- ELF5 isoform expression is tissue-specific and significantly altered in cancer.Breast cancer research : BCR · 2016Article
- ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells.PLoS biology · 2015Article
- Metabolite profiling stratifies pancreatic ductal adenocarcinomas into subtypes with distinct sensitivities to metabolic inhibitors.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.Nature cell biology · 2015Article
- An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth.Genes & development · 2014Article
- A high-content cellular senescence screen identifies candidate tumor suppressors, including EPHA3.Cell cycle (Georgetown, Tex.) · 2013Article
- Three-dimensional imaging of solvent-cleared organs using 3DISCO.Nature protocols · 2012Article
- ELF5 suppresses estrogen sensitivity and underpins the acquisition of antiestrogen resistance in luminal breast cancer.PLoS biology · 2012Article
- A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Interaction between FIP5 and SNX18 regulates epithelial lumen formation.The Journal of cell biology · 2011Article
- p21-activated kinase 1: PAK'ed with potential.Oncotarget · 2011Review
- The antiproliferative effects of progestins in T47D breast cancer cells are tempered by progestin induction of the ETS transcription factor Elf5.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Ubiquitin hydrolase Dub3 promotes oncogenic transformation by stabilizing Cdc25A.Nature cell biology · 2010Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be independently engineered into the target system, resulting in experimental delay and an increased potential for selection of a cell subpopulation that differs significantly from the parental line. We have therefore developed pHUSH, an inducible expression system that allows regulated expression of shRNA, miRNA or cDNA cassettes on a single viral vector.
resultsBoth Pol II and Pol III promoters have been successfully combined with a second expression cassette containing a codon-optimized tetracycline repressor and selectable marker. We provide examples of how pHUSH has been successfully employed to study the function of target genes in a number of cell types within in vitro and in vivo assays, including conditional gene knockdown in a murine model of brain cancer.
conclusionWe have successfully developed and employed a single vector system that enables Doxycycline regulated RNAi or transgene expression in a variety of in vitro and in vivo model systems. These studies demonstrate the broad application potential of pHUSH for conditional genetic engineering in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.