ArticlePloS one2007
Identification of upper respiratory tract pathogens using electrochemical detection on an oligonucleotide microarray.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Microbiome of the paranasal sinuses: Update and literature review.American journal of rhinology & allergyPooled it
- The potential of electrochemistry for the detection of coronavirus-induced infections.Trends in analytical chemistry : TRAC · 2020Review
- Antibody Modified Gold Electrode as an Impedimetric Biosensor for the Detection ofSensors (Basel, Switzerland) · 2020Article
- Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait.Journal of medical virology · 2018Article
- A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.Oncotarget · 2017Article
- Mycoplasma pneumoniae from the Respiratory Tract and Beyond.Clinical microbiology reviews · 2017Review
- Direct multiplexed whole genome sequencing of respiratory tract samples reveals full viral genomic information.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Evolving gene targets and technology in influenza detection.Molecular diagnosis & therapy · 2013Review
- Comparison of a multiplex real-time PCR assay with a multiplex Luminex assay for influenza virus detection.Journal of clinical microbiology · 2013Article
- Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.PLoS neglected tropical diseases · 2013Article
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
- Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.The Journal of molecular diagnostics : JMD · 2012Review
- Comparative genomics of Helicobacter pylori strains of China associated with different clinical outcome.PloS one · 2012Article
- Development of adenoviral vector-based mucosal vaccine against influenza.Journal of molecular medicine (Berlin, Germany) · 2011Review
- Seasonal changes in bacterial and archaeal gene expression patterns across salinity gradients in the Columbia River coastal margin.PloS one · 2010Article
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- A low density oligonucleotide microarray for the detection of viral and atypical bacterial respiratory pathogens.Journal of virological methods · 2010Article
- Design and validation of a microarray for detection, hemagglutinin subtyping, and pathotyping of avian influenza viruses.Journal of clinical microbiology · 2009Article
- Applications of microarrays in pathogen detection and biodefence.Trends in biotechnology · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial and viral upper respiratory infections (URI) produce highly variable clinical symptoms that cannot be used to identify the etiologic agent. Proper treatment, however, depends on correct identification of the pathogen involved as antibiotics provide little or no benefit with viral infections. Here we describe a rapid and sensitive genotyping assay and microarray for URI identification using standard amplification and hybridization techniques, with electrochemical detection (ECD) on a semiconductor-based oligonucleotide microarray. The assay was developed to detect four bacterial pathogens (Bordetella pertussis, Streptococcus pyogenes, Chlamydia pneumoniae and Mycoplasma pneumoniae) and 9 viral pathogens (adenovirus 4, coronavirus OC43, 229E and HK, influenza A and B, parainfluenza types 1, 2, and 3 and respiratory syncytial virus. This new platform forms the basis for a fully automated diagnostics system that is very flexible and can be customized to suit different or additional pathogens. Multiple probes on a flexible platform allow one to test probes empirically and then select highly reactive probes for further iterative evaluation. Because ECD uses an enzymatic reaction to create electrical signals that can be read directly from the array, there is no need for image analysis or for expensive and delicate optical scanning equipment. We show assay sensitivity and specificity that are excellent for a multiplexed format.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.