ArticleRNA (New York, N.Y.)2007
Selective quenching of fluorescence from unbound oligonucleotides by gold nanoparticles as a probe of RNA structure.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A Review on Recent Trends in Green Synthesis of Gold Nanoparticles for Tuberculosis.Advanced pharmaceutical bulletin · 2021Review
- Two colorimetric ampicillin sensing schemes based on the interaction of aptamers with gold nanoparticles.Mikrochimica acta · 2019Article
- The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling.Journal of nanobiotechnology · 2017Article
- Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays.Analytical chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Binding of small oligonucleotides to the periphery of folded RNA can provide insight into the secondary structure of complex RNA in solution. To discriminate between bound and unbound fluorescein-labeled 2'-O-methyl RNA probes, we use ionically coated gold nanoparticles to selectively adsorb unbound probes and quench their fluorescence. The target is the 3' untranslated region of Bombyx mori R2 RNA. Fluorescence indicates that R2 sequences complementary to some of the probes are accessible for binding in the three-dimensional structure. Hybridization occurs under homogeneous conditions in the absence of the gold nanoparticles so that steric issues associated with chip-based assays are avoided. The assay is compatible with well plate formats, takes less than 5 min, and requires only 2 pmol or less of unlabeled target RNA per probe sequence tested.
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