ArticleProceedings of the National Academy of Sciences of the United States of America2007
Inner ear hair cells produced in vitro by a mesenchymal-to-epithelial transition.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed.
- Postnatal Changes of Neural Stem Cells in the Mammalian Auditory Cortex.International journal of molecular sciences · 2021Article
- Stepwise Induction of Inner Ear Hair Cells From Mouse Embryonic Fibroblasts via Mesenchymal- to-Epithelial Transition and Formation of Otic Epithelial Cells.Frontiers in cell and developmental biology · 2021Article
- Role of the periotic mesenchyme in the development of sensory cells in early mouse cochlea.Journal of otology · 2020Article
- Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation.International journal of molecular sciences · 2020Article
- Research progress on flat epithelium of the inner ear.Physiological research · 2020Review
- Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.Stem cells and development · 2019Article
- Article
- Epigenetic DNA Demethylation Causes Inner Ear Stem Cell Differentiation into Hair Cell-Like Cells.Frontiers in cellular neuroscience · 2016Article
- Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells.Stem cells international · 2016Article
- C-MYC transcriptionally amplifies SOX2 target genes to regulate self-renewal in multipotent otic progenitor cells.Stem cell reports · 2015Article
- Inner ear hair cell-like cells from human embryonic stem cells.Stem cells and development · 2014Article
- Article
- Induction of inner ear hair cell-like cells from Math1-transfected mouse ES cells.Cell death & disease · 2013Article
- The potential of stem cells for the restoration of auditory function in humans.Regenerative medicine · 2013Review
- Generation of human inner ear prosensory-like cells via epithelial-to-mesenchymal transition.Regenerative medicine · 2012Article
- Sensory epithelial cells acquire features of prosensory cells via epithelial to mesenchymal transition.Stem cells and development · 2012Article
- In vitro differentiation of mouse embryonic stem cells into inner ear hair cell-like cells using stromal cell conditioned medium.Cell death & disease · 2012Article
- Hypotonicity-induced TRPV4 function in renal collecting duct cells: modulation by progressive cross-talk with Ca2+-activated K+ channels.Cell calcium · 2012Article
- MYC gene delivery to adult mouse utricles stimulates proliferation of postmitotic supporting cells in vitro.PloS one · 2012Article
- Axonemal positioning and orientation in three-dimensional space for primary cilia: what is known, what is assumed, and what needs clarification.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Review
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2 authors.
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Abstract
Sensory hair cell loss is a major contributor to disabling hearing and balance deficits that affect >250 million people worldwide. Sound exposures, infections, drug toxicity, genetic disorders, and aging all can cause hair cell loss and lead to permanent sensory deficits. Progress toward treatments for these deficits has been limited, in part because hair cells have only been obtainable via microdissection of the anatomically complex internal ear. Attempts to produce hair cells in vitro have resulted in reports of some success but have required transplantation into embryonic ears or coculturing with other tissues. Here, we show that avian inner ear cells can be cultured and passaged for months, frozen, and expanded to large numbers without other tissues. At any point from passage 6 up to at least passage 23, these cultures can be fully dissociated and then aggregated in suspension to induce a mesenchymal-to-epithelial transition that reliably yields new polarized sensory epithelia. Those epithelia develop numerous hair cells that are crowned by hair bundles, composed of a single kinocilium and an asymmetric array of stereocilia. These hair cells exhibit rapid permeance to FM1-43, a dye that passes through open mechanotransducing channels. Because a vial of frozen cells can now provide the capacity to produce bona fide hair cells completely in vitro, these discoveries should open new avenues of research that may ultimately contribute to better treatments for hearing loss and other inner ear disorders.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.