ArticleProtein science : a publication of the Protein Society2007
The Bacillus licheniformis BlaP beta-lactamase as a model protein scaffold to study the insertion of protein fragments.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- The Right-Handed Parallel β-Helix Topology ofBiomolecules · 2021Article
- Reflections on professor Sir Christopher M. Dobson (1949-2019).Biophysical reviews · 2020Article
- Equilibrium partially folded states of B. licheniformisEuropean biophysics journal : EBJ · 2019Article
- The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions.Journal of visualized experiments : JoVE · 2018Article
- In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model.Journal of negative results in biomedicine · 2017Article
- An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.BMC biotechnology · 2017Article
- Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who's Leading HCHT's Biological Function.Scientific reports · 2017Article
- Class A β-lactamases as versatile scaffolds to create hybrid enzymes: applications from basic research to medicine.BioMed research international · 2013Review
- Article
- Comparative functional analysis of the human macrophage chitotriosidase.Protein science : a publication of the Protein Society · 2011Article
- Epitope mapping and key amino acid identification of anti-CD22 immunotoxin CAT-8015 using hybrid β-lactamase display.Protein engineering, design & selection : PEDS · 2011Article
- More than 200 genes required for methane formation from H₂ and CO₂ and energy conservation are present in Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus.Archaea (Vancouver, B.C.) · 2011Article
- Directed evolution of recombinase specificity by split gene reassembly.Nucleic acids research · 2010Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using genetic engineering technologies, the chitin-binding domain (ChBD) of the human macrophage chitotriosidase has been inserted into the host protein BlaP, a class A beta-lactamase produced by Bacillus licheniformis. The product of this construction behaved as a soluble chimeric protein that conserves both the capacity to bind chitin and to hydrolyze beta-lactam moiety. Here we describe the biochemical and biophysical properties of this protein (BlaPChBD). This work contributes to a better understanding of the reciprocal structural and functional effects of the insertion on the host protein scaffold and the heterologous structured protein fragments. The use of BlaP as a protein carrier represents an efficient approach to the functional study of heterologous protein fragments.
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