ArticleTraffic (Copenhagen, Denmark)2007
Structural features of vps35p involved in interaction with other subunits of the retromer complex.
Article in Traffic (Copenhagen, Denmark), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- VPS35 and retromer dysfunction in Parkinson's disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Receptor Recycling by Retromer.Molecular and cellular biology · 2023Review
- Updated Insight into the Physiological and Pathological Roles of the Retromer Complex.International journal of molecular sciences · 2017Review
- Article
- Biogenesis of endosome-derived transport carriers.Cellular and molecular life sciences : CMLS · 2015Review
- Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy.Nature communications · 2014Article
- RME-8 coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation.Journal of cell science · 2014Article
- Mechanisms governing the endosomal membrane recruitment of the core retromer in Arabidopsis.The Journal of biological chemistry · 2013Article
- Rab GTPase regulation of retromer-mediated cargo export during endosome maturation.Molecular biology of the cell · 2012Article
- Physiology and pathology of endosome-to-Golgi retrograde sorting.Traffic (Copenhagen, Denmark) · 2011Review
- env1 Mutant of VPS35 gene exhibits unique protein localization and processing phenotype at Golgi and lysosomal vacuole in Saccharomyces cerevisiae.Molecular and cellular biochemistry · 2011Article
- Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome.Molecular biology of the cell · 2008Article
- Retromer.Current opinion in cell biology · 2008Review
- Retrograde traffic in the biosynthetic-secretory route.Histochemistry and cell biology · 2008Review
- Dominant-negative behavior of mammalian Vps35 in yeast requires a conserved PRLYL motif involved in retromer assembly.Traffic (Copenhagen, Denmark) · 2007Article
- Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex.The Biochemical journal · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The penta-subunit retromer complex of yeast mediates selective retrieval of membrane proteins from the prevacuolar endosome to the trans Golgi network. In this study, we set out to generate a panel of vps35 dominant-negative mutants that disrupt retromer-mediated cargo sorting. Mapping of the mutations revealed two types of alterations leading to dominant-negative behavior of the 944-amino acid protein: (i) mutations at or near the R(98) residue or (ii) C-terminal truncations exemplified by a nonsense mutation at codon 733. Both could be suppressed by overexpression of wild-type Vps35p, suggesting that these dominant-negative mutants compete for interactions with other retromer subunits. Interestingly, Vps35-R(98)W expression destabilized Vps26p while having no effect on Vps29p stability, while Vps35-Q(733)* expression affected Vps29p stability but had no effect on Vps26p. Measurement of Vps35/Vps26 and Vps35/Vps29 pairwise associations by coimmunoprecipitation in the presence or absence of other retromer subunits indicated that the R(98) residue, which is part of a conserved PRLYL motif, is critical for Vps35p binding to Vps26p, while both R(98) and residues 733-944 are needed for efficient binding to Vps29p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.