ArticleMolecular microbiology2007
Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12.
Article in Molecular microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 83 citations in OpenAlex.
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- The evolution of MarR family transcription factors as counter-silencers in regulatory networks.Current opinion in microbiology · 2020Review
- SlyA Transcriptional Regulator Is Not Directly Affected by ppGpp Levels.Frontiers in microbiology · 2020Article
- NusG prevents transcriptional invasion of H-NS-silenced genes.PLoS genetics · 2019Article
- SlyA and HilD Counteract H-NS-Mediated Repression on theJournal of bacteriology · 2019Article
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- Endogenous and Foreign Nucleoid-Associated Proteins of Bacteria: Occurrence, Interactions and Effects on Mobile Genetic Elements and Host's Biology.Computational and structural biotechnology journal · 2019Review
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- Control of virulence gene transcription by indirect readout in Vibrio cholerae and Salmonella enterica serovar Typhimurium.Environmental microbiology · 2017Review
- LdtR is a master regulator of gene expression in Liberibacter asiaticus.Microbial biotechnology · 2017Article
- Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon.Microbiology (Reading, England) · 2017Article
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- SlyA regulates phytotoxin production and virulence in Dickeya zeae EC1.Molecular plant pathology · 2016Article
- Screening of genes involved in interactions with intestinal epithelial cells in Cronobacter sakazakii.AMB Express · 2016Article
- Molecular Characterization of the Vacuolating Autotransporter Toxin in Uropathogenic Escherichia coli.Journal of bacteriology · 2016Article
- Characterization of SlyA in Shigella flexneri Identifies a Novel Role in Virulence.Infection and immunity · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Haemolysin E is a cytolytic pore-forming toxin found in several Escherichia coli and Salmonella enterica strains. Expression of hlyE is repressed by the global regulator H-NS (histone-like nucleoid structuring protein), but can be activated by the regulator SlyA. Expression of a chromosomal hlyE-lacZ fusion in an E. coli slyA mutant was reduced to 60% of the wild-type level confirming a positive role for SlyA. DNase I footprint analysis revealed the presence of two separate SlyA binding sites, one located upstream, the other downstream of the hlyE transcriptional start site. These sites overlap AT-rich H-NS binding sites. Footprint and gel shift data showed that whereas H-NS prevented binding of RNA polymerase (RNAP) at the hlyE promoter (PhlyE), SlyA allowed binding of RNAP, but inhibited binding of H-NS. Accordingly, in vitro transcription analyses showed that addition of SlyA protein relieved H-NS-mediated repression of hlyE. Based on these observations a model for SlyA/H-NS regulation of hlyE expression is proposed in which the relative concentrations of SlyA and H-NS govern the nature of the nucleoprotein complexes formed at PhlyE. When H-NS is dominant RNAP binding is inhibited and hlyE expression is silenced; when SlyA is dominant H-NS binding is inhibited allowing RNAP access to the promoter facilitating hlyE transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.