ArticleBiophysical journal2008
Long-range nonanomalous diffusion of quantum dot-labeled aquaporin-1 water channels in the cell plasma membrane.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas.Scientific reports · 2021Article
- A Novel Biotinylated Homotryptamine Derivative for Quantum Dot Imaging of Serotonin Transporter in Live Cells.Frontiers in cellular neuroscience · 2021Article
- Review
- Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion.Science (New York, N.Y.) · 2019Article
- Quantum dots reveal heterogeneous membrane diffusivity and dynamic surface density polarization of dopamine transporter.PloS one · 2019Article
- Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells.Biomolecules · 2018Article
- Quantum dots for quantitative imaging: from single molecules to tissue.Cell and tissue research · 2015Review
- Article
- Wanted: scalable tracers for diffusion measurements.The journal of physical chemistry. B · 2014Review
- Actin cytoskeleton reorganization by Syk regulates Fcγ receptor responsiveness by increasing its lateral mobility and clustering.Developmental cell · 2014Article
- Lateral mobility of L-type calcium channels in synaptic terminals of retinal bipolar cells.Molecular vision · 2013Article
- Reduced PDZ interactions of rescued ΔF508CFTR increases its cell surface mobility.The Journal of biological chemistry · 2012Article
- Quantum dots: synthesis, bioapplications, and toxicity.Nanoscale research letters · 2012Article
- Light inactivation of water transport and protein-protein interactions of aquaporin-Killer Red chimeras.The Journal of general physiology · 2012Article
- Live-cell imaging of aquaporin-4 supramolecular assembly and diffusion.Methods in enzymology · 2012Review
- Confinement of β(1)- and β(2)-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae.Molecular biology of the cell · 2011Article
- Aquaporin-4: orthogonal array assembly, CNS functions, and role in neuromyelitis optica.Acta pharmacologica Sinica · 2011Review
- Water flux through human aquaporin 1: inhibition by intracellular furosemide and maximal response with high osmotic gradients.European biophysics journal : EBJ · 2011Article
- Binding affinity and specificity of neuromyelitis optica autoantibodies to aquaporin-4 M1/M23 isoforms and orthogonal arrays.The Journal of biological chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aquaporin-1 (AQP1) is an integral membrane protein that facilitates osmotic water transport across cell plasma membranes in epithelia and endothelia. AQP1 has no known specific interactions with cytoplasmic or membrane proteins, but its recovery in a detergent-insoluble membrane fraction has suggested possible raft association. We tracked the membrane diffusion of AQP1 molecules labeled with quantum dots at an engineered external epitope at frame rates up to 91 Hz and over times up to 6 min. In transfected COS-7 cells, >75% of AQP1 molecules diffused freely over approximately 7 mum in 5 min, with diffusion coefficient, D(1-3) approximately 9 x 10(-10) cm(2)/s. In MDCK cells, approximately 60% of AQP1 diffused freely, with D(1-3) approximately 3 x 10(-10) cm(2)/s. The determinants of AQP1 diffusion were investigated by measurements of AQP1 diffusion following skeletal disruption (latrunculin B), lipid/raft perturbations (cyclodextrin and sphingomyelinase), and bleb formation. We found that cytoskeletal disruption had no effect on AQP1 diffusion in the plasma membrane, but that diffusion was increased greater than fourfold in protein de-enriched blebs. Cholesterol depletion in MDCK cells greatly restricted AQP1 diffusion, consistent with the formation of a network of solid-like barriers in the membrane. These results establish the nature and determinants of AQP1 diffusion in cell plasma membranes and demonstrate long-range nonanomalous diffusion of AQP1, challenging the prevailing view of universally anomalous diffusion of integral membrane proteins, and providing evidence against the accumulation of AQP1 in lipid rafts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.