ArticleBiophysical journal2008
Enhancement of L-selectin, but not P-selectin, bond formation frequency by convective flow.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Binding of Soluble Ligands to Membrane Receptors: A Molecular Dynamics Simulation Study.The journal of physical chemistry. B · 2025Article
- Immunomodulation by endothelial cells - partnering up with the immune system?Nature reviews. Immunology · 2022Review
- TCR-pMHC kinetics under force in a cell-free system show no intrinsic catch bond, but a minimal encounter duration before binding.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Contractile fibers and catch-bond clusters: a biological force sensor?Biophysical journal · 2013Article
- Rupture of single receptor-ligand bonds: a new insight into probability distribution function.Colloids and surfaces. B, Biointerfaces · 2013Article
- A High-Throughput Technique Reveals the Load- and Site Density-Dependent Kinetics of E-Selectin.Cellular and molecular bioengineering · 2012Article
- Effect of loading conditions on the dissociation behaviour of catch bond clusters.Journal of the Royal Society, Interface · 2012Article
- Multivalent binding of nanocarrier to endothelial cells under shear flow.Biophysical journal · 2011Article
- Leukocyte rolling on engineered nanodot surfaces.Micro & nano letters · 2011Article
- The Effects of Load on E-Selectin Bond Rupture and Bond Formation.Cellular and molecular bioengineering · 2010Article
- Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.PLoS computational biology · 2009Article
- Action at a distance: lengthening adhesion bonds with poly(ethylene glycol) spacers enhances mechanically stressed affinity for improved vascular targeting of microparticles.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Biomolecule association rates do not provide a complete description of bond formation.Biophysical journal · 2009Article
- Surface-bound selectin-ligand binding is regulated by carrier diffusion.European biophysics journal : EBJ · 2009Article
- Selectin catch-slip kinetics encode shear threshold adhesive behavior of rolling leukocytes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Studying Molecular Interactions at the Single Bond Level with a Laminar Flow Chamber.Cellular and molecular bioengineering · 2008Article
- Catch strip assay for the relative assessment of two-dimensional protein association kinetics.Analytical chemistry · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
L-selectin-mediated leukocyte rolling has been proposed to require a high rate of bond formation compared to that of P-selectin to compensate for its much higher off-rate. To test this hypothesis, a microbead system was utilized to measure relative L-selectin and P-selectin bond formation rates on their common ligand P-selectin glycoprotein ligand-1 (PSGL-1) under shear flow. Using video microscopy, we tracked selectin-coated microbeads to detect the formation frequency of adhesive tether bonds. From velocity distributions of noninteracting and interacting microbeads, we observed that tether bond formation rates for P-selectin on PSGL-1 decreased with increasing wall shear stress, from 0.14 +/- 0.04 bonds/microm at 0.2 dyn/cm(2) to 0.014 +/- 0.003 bonds/microm at 1.0 dyn/cm(2). In contrast, L-selectin tether bond formation increased from 0.017 +/- 0.005 bonds/microm at 0.2 dyn/cm(2) to 0.031 +/- 0.005 bonds/microm at 1.0 dyn/cm(2). L-selectin tether bond formation rates appeared to be enhanced by convective transport, whereas P-selectin rates were inhibited. The transition force for the L-selectin catch-slip transition of 44 pN/bond agreed well with theoretical models (Pereverzev et al. 2005. Biophys. J. 89:1446-1454). Despite catch bond behavior, hydrodymanic shear thresholding was not detected with L-selectin beads rolling on PSGL-1. We speculate that shear flow generated compressive forces may enhance L-selectin bond formation relative to that of P-selectin and that L-selectin bonds with PSGL-1 may be tuned for the compressive forces characteristic of leukocyte-leukocyte collisions during secondary capture on the blood vessel wall. This is the first report, to our knowledge, comparing L-selectin and P-selectin bond formation frequencies in shear flow.
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