ArticleGenetics2007
Disruption of topoisomerase II perturbs pairing in drosophila cell culture.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 61 citations in OpenAlex.
- Drosophila ring chromosomes interact with sisters and homologs to produce anaphase bridges in mitosis.Genetics · 2024Article
- The zinc-finger protein Z4 cooperates with condensin II to regulate somatic chromosome pairing and 3D chromatin organization.Nucleic acids research · 2024Article
- Fruit fly for anticancer drug discovery and repurposing.Annals of medicine and surgery (2012) · 2023Article
- The 3D genome landscape: Diverse chromosomal interactions and their functional implications.Frontiers in cell and developmental biology · 2022Review
- Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Fidelity of Mechanisms Governing the Cell Cycle.Results and problems in cell differentiation · 2022Article
- Mitotic Antipairing of Homologous Chromosomes.Results and problems in cell differentiation · 2022Article
- Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype.Results and problems in cell differentiation · 2022Review
- Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila.Cell death & disease · 2021Article
- Oligopaint DNA FISH reveals telomere-based meiotic pairing dynamics in the silkworm, Bombyx mori.PLoS genetics · 2021Article
- Multi-Scale Organization of theGenes · 2021Review
- Pericentromeric heterochromatin is hierarchically organized and spatially contacts H3K9me2 islands in euchromatin.PLoS genetics · 2020Article
- Position Effects Influence Transvection inGenetics · 2019Article
- Highly structured homolog pairing reflects functional organization of the Drosophila genome.Nature communications · 2019Article
- Recurrent Losses and Rapid Evolution of the Condensin II Complex in Insects.Molecular biology and evolution · 2019Article
- Polymer coil-globule phase transition is a universal folding principle of Drosophila epigenetic domains.Epigenetics & chromatin · 2019Article
- Condensin II Counteracts Cohesin and RNA Polymerase II in the Establishment of 3D Chromatin Organization.Cell reports · 2019Article
- Condensin II drives large-scale folding and spatial partitioning of interphase chromosomes in Drosophila nuclei.PLoS genetics · 2018Article
- Polyteny: still a giant player in chromosome research.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Review
- Article
- Interallelic Transcriptional Enhancement as an in Vivo Measure of Transvection in Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Homolog pairing refers to the alignment and physical apposition of homologous chromosomal segments. Although commonly observed during meiosis, homolog pairing also occurs in nonmeiotic cells of several organisms, including humans and Drosophila. The mechanism underlying nonmeiotic pairing, however, remains largely unknown. Here, we explore the use of established Drosophila cell lines for the analysis of pairing in somatic cells. Using fluorescent in situ hybridization (FISH), we assayed pairing at nine regions scattered throughout the genome of Kc167 cells, observing high levels of homolog pairing at all six euchromatic regions assayed and variably lower levels in regions in or near centromeric heterochromatin. We have also observed extensive pairing in six additional cell lines representing different tissues of origin, different ploidies, and two different species, demonstrating homolog pairing in cell culture to be impervious to cell type or culture history. Furthermore, by sorting Kc167 cells into G1, S, and G2 subpopulations, we show that even progression through these stages of the cell cycle does not significantly change pairing levels. Finally, our data indicate that disrupting Drosophila topoisomerase II (Top2) gene function with RNAi and chemical inhibitors perturbs homolog pairing, suggesting Top2 to be a gene important for pairing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.