ArticleApplied and environmental microbiology2007
Multiplex assay based on single-nucleotide polymorphisms for rapid identification of Brucella isolates at the species level.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- The ftsA gene as a molecular marker for phylogenetic studies in Bradyrhizobium and identification of Bradyrhizobium japonicum.Journal of applied genetics · 2019Article
- Brucella neotomae Infection in Humans, Costa Rica.Emerging infectious diseases · 2017Article
- Article
- Differential diagnosis of Brucella abortus by real-time PCR based on a single-nucleotide polymorphisms.The Journal of veterinary medical science · 2016Article
- Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the GenusFrontiers in microbiology · 2016Article
- Brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts.Infection and immunity · 2015Article
- Article
- Article
- A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata).Veterinary research · 2011Review
- A species independent universal bio-detection microarray for pathogen forensics and phylogenetic classification of unknown microorganisms.BMC microbiology · 2011Article
- High throughput MLVA-16 typing for Brucella based on the microfluidics technology.BMC microbiology · 2011Article
- Review of detection of Brucella spp. by polymerase chain reaction.Croatian medical journal · 2010Review
- Development of a multiplex primer extension assay for rapid detection of Salmonella isolates of diverse serotypes.Journal of clinical microbiology · 2010Article
- Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.Journal of clinical microbiology · 2010Article
- Diversity and distribution of anaeromyxobacter strains in a uranium-contaminated subsurface environment with a nonuniform groundwater flow.Applied and environmental microbiology · 2009Article
- Evaluation of single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities.Applied and environmental microbiology · 2009Article
- Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis.BMC microbiology · 2009Article
- Whole-genome-based phylogeny and divergence of the genus Brucella.Journal of bacteriology · 2009Article
- Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis.BMC microbiology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genus Brucella includes a number of species that are major animal pathogens worldwide and significant causes of zoonotic infections of humans. Traditional methods of identifying Brucella to the species level can be time-consuming, can be subjective, and can pose a hazard to laboratory personnel in the absence of suitable biocontainment facilities. Using a robust phylogenetic framework, a number of single-nucleotide polymorphisms (SNPs) that define particular species within the genus were identified. These SNPs were used to develop a multiplex SNP detection assay, based on primer extension technology, that can rapidly and unambiguously identify an isolate as a member of one of the six classical Brucella species or as a member of the recently identified marine mammal group.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.