ArticleProceedings of the National Academy of Sciences of the United States of America2007
Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Mechanisms and proteins involved in long-distance interactions.Frontiers in genetics · 2014Review
- Mitotic bookmarking by transcription factors.Epigenetics & chromatin · 2013Article
- The role of chromatin insulators in nuclear architecture and genome function.Current opinion in genetics & development · 2013Review
- Article
- Enhancers and silencers: an integrated and simple model for their function.Epigenetics & chromatin · 2012Article
- Do chromatin loops provide epigenetic gene expression states?Current opinion in genetics & development · 2010Review
- A mitotic transcriptional switch in polycystic kidney disease.Nature medicine · 2010Article
- CTCF: master weaver of the genome.Cell · 2009Review
- Chromatin insulators: regulatory mechanisms and epigenetic inheritance.Molecular cell · 2008Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During mitosis in higher eukaryotic cells, transcription is silenced and transcription complexes are absent from promoters in the condensed chromosomes; however, epigenetic information concerning the pattern of expressed and silent genes must be preserved. Recently, it has been reported that CTCF, a major protein in vertebrate insulator elements, remains associated with mitotic chromatin. If the structure of insulators is preserved during mitosis, then it is possible that insulators can function as components or elements of the mechanism involved in the transfer of epigenetic information through the mitotic phase and can help guide the reconstitution of domain structure and nuclear organization after the completion of this phase. We have studied the chromatin structure of the insulator upstream of the c-MYC gene in mitotic HeLa cells. The region of the insulator corresponds to the DNase I hypersensitive site I, but Southern blot analysis revealed that hypersensitivity was lost during mitosis. High resolution in vivo footprinting analysis using dimethyl sulfate, UV light, psoralen, and DNase I also demonstrated the disappearance of the sequence-specific direct binding of CTCF and the absence of detectable structures during mitosis. Thus, it appears that the nucleoprotein complex involving this insulator element must be reassembled de novo with each new cell generation.
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