ArticleBMC plant biology2007
Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum.
Article in BMC plant biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 134 citations in OpenAlex.
- Multimodal Inhibition ofJournal of agricultural and food chemistry · 2025Article
- Uncovering functional deterioration in the rhizosphere microbiome associated with post-green revolution wheat cultivars.Environmental microbiome · 2025Article
- Effects of Bacillus in Pectobacterium quorum quenching: A survey of two different acyl-homoserine lactonases.Folia microbiologica · 2024Article
- Selection and validation of reference genes suitable for gene expression analysis by Reverse Transcription Quantitative real-time PCR in Acinetobacter baumannii.Scientific reports · 2024Article
- Inhibition of AcrAB-TolC enhances antimicrobial activity of phytochemicals inFrontiers in plant science · 2023Article
- Inhibitory effects ofJournal of dental sciences · 2023Article
- The RNA-Binding Protein ProQ Impacts Exopolysaccharide Biosynthesis and Second Messenger Cyclic di-GMP Signaling in the Fire Blight Pathogen Erwinia amylovora.Applied and environmental microbiology · 2022Article
- A comparative study on the genomes, transcriptomes, and metabolic properties ofEngineering microbiology · 2022Article
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- Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis inFrontiers in veterinary science · 2022Article
- YciR, a Specific 3'-Phosphodiesterase, Plays a Role in the Pathogenesis of UropathogenicFrontiers in microbiology · 2022Article
- Pectobacterium atrosepticum KDPG aldolase, Eda, participates in the Entner-Doudoroff pathway and independently inhibits expression of virulence determinants.Molecular plant pathology · 2021Article
- Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness ofFrontiers in plant science · 2021Article
- Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis.MicrobiologyOpen · 2019Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions.Extremophiles : life under extreme conditions · 2019Article
- Delineation of the functional and structural properties of the glutathione transferase family from the plant pathogen Erwinia carotovora.Functional & integrative genomics · 2019Article
- Interaction-Specific Changes in the Transcriptome ofFrontiers in microbiology · 2019Article
- Functional Characterization of a Global Virulence Regulator Hfq and Identification of Hfq-Dependent sRNAs in the Plant PathogenFrontiers in microbiology · 2019Article
- Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR.Scientific reports · 2018Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReal-time RT-PCR has become a powerful technique to monitor low-abundance mRNA expression and is a useful tool when examining bacterial gene expression inside infected host tissues. However, correct evaluation of data requires accurate and reliable normalisation against internal standards. Thus, the identification of reference genes whose expression does not change during the course of the experiment is of paramount importance. Here, we present a study where manipulation of cultural growth conditions and in planta experiments have been used to validate the expression stability of reference gene candidates for the plant pathogen Pectobacterium atrosepticum, belonging to the family Enterobacteriaceae.
resultsOf twelve reference gene candidates tested, four proved to be stably expressed both in six different cultural growth conditions and in planta. Two of these genes (recA and ffh), encoding recombinase A and signal recognition particle protein, respectively, proved to be the most stable set of reference genes under the experimental conditions used. In addition, genes proC and gyrA, encoding pyrroline-5-carboxylate reductase and DNA gyrase, respectively, also displayed relatively stable mRNA expression levels.
conclusionBased on these results, we suggest recA and ffh as suitable candidates for accurate normalisation of real-time RT-PCR data for experiments investigating the plant pathogen P. atrosepticum and potentially other related pathogens.
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