ArticlePlant physiology2007
Alteration of organic acid metabolism in Arabidopsis overexpressing the maize C4 NADP-malic enzyme causes accelerated senescence during extended darkness.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 112 citations in OpenAlex.
- Overexpression of cytosolic NADP-malic enzyme 1 from the common ice plant enhances water-deficit and high-light stress tolerance by modulating water-use efficiency and flavonoid biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
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- Overexpression of chloroplastic Zea mays NADP-malic enzyme (ZmNADP-ME) confers tolerance to salt stress in Arabidopsis thaliana.Photosynthesis research · 2023Article
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Shedding Light on the Dynamic Role of the "Target of Rapamycin" Kinase in the Fast-Growing CFrontiers in plant science · 2021Article
- Ectopic expression of CPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Review
- Physiological Profiling of Embryos and Dormant Seeds in TwoFrontiers in plant science · 2020Article
- Posttranslational Modification of the NADP-Malic Enzyme Involved in CThe Plant cell · 2019Article
- Laying the Foundation for Crassulacean Acid Metabolism (CAM) Biodesign: Expression of the CFrontiers in plant science · 2019Article
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- A robust circadian rhythm of metabolites in Arabidopsis thaliana mutants with enhanced growth characteristics.PloS one · 2019Article
- A Glycine soja group S2 bZIP transcription factor GsbZIP67 conferred bicarbonate alkaline tolerance in Medicago sativa.BMC plant biology · 2018Article
- The NAC Transcription Factor SlNAP2 Regulates Leaf Senescence and Fruit Yield in Tomato.Plant physiology · 2018Article
- Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.Scientific reports · 2018Article
- Modifications in Organic Acid Profiles During Fruit Development and Ripening: Correlation or Causation?Frontiers in plant science · 2018Review
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- Impaired Malate and Fumarate Accumulation Due to the Mutation of the Tonoplast Dicarboxylate Transporter Has Little Effects on Stomatal Behavior.Plant physiology · 2017Article
- Intergrative metabolomic and transcriptomic analyses unveil nutrient remobilization events in leaf senescence of tobacco.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The full-length cDNA encoding the maize (Zea mays) C(4) NADP-malic enzyme was expressed in Arabidopsis (Arabidopsis thaliana) under the control of the cauliflower mosaic virus 35S promoter. Homozygous transgenic plants (MEm) were isolated with activities ranging from 6- to 33-fold of those found in the wild type. The transformants did not show any differences in morphology and development when grown in long days; however, dark-induced senescence progressed more rapidly in MEm plants compared to the wild type. Interestingly, senescence could be retarded in the transgenic lines by exogenously supplying glucose, sucrose, or malate, suggesting that the lack of a readily mobilized carbon source is likely to be the initial factor leading to the premature induction of senescence in MEm plants. A comprehensive metabolic profiling on whole rosettes allowed determination of approximately 80 metabolites during a diurnal cycle as well as following dark-induced senescence and during metabolic complementation assays. MEm plants showed no differences in the accumulation and degradation of carbohydrates with respect to the wild type in all conditions tested, but accumulated lower levels of intermediates used as respiratory substrates, prominently malate and fumarate. The data indicated that extremely low levels of malate and fumarate are responsible for the accelerated dark-induced senescence encountered in MEm plants. Thus, in prolonged darkness these metabolites are consumed faster than in the wild type and, as a consequence, MEm plants enter irreversible senescence more rapidly. In addition, the data revealed that both malate and fumarate are important forms of fixed carbon that can be rapidly metabolized under stress conditions in Arabidopsis.
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