ArticleBMC biotechnology2007
A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.Biomedical microdevices · 2026Article
- Activation of cell migration via morphological changes in focal adhesions depends on shear stress in MYCN-amplified neuroblastoma cells.Journal of the Royal Society, Interface · 2019Article
- Quantitative analysis of sensitivity to a Wnt3a gradient in determination of the pole-to-pole axis of mitotic cells by using a microfluidic device.FEBS open bio · 2018Article
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- Neural Stem Cell Differentiation Using Microfluidic Device-Generated Growth Factor Gradient.Biomolecules & therapeutics · 2018Article
- Accelerated Biofluid Filling in Complex Microfluidic Networks by Vacuum-Pressure Accelerated Movement (V-PAM).Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device.Stem cell research & therapy · 2016Article
- Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry.Biomicrofluidics · 2015Article
- Article
- Micro- and nanoengineering for stem cell biology: the promise with a caution.Trends in biotechnology · 2011Review
- A modular cell culture device for generating arrays of gradients using stacked microfluidic flows.Biomicrofluidics · 2011Article
- Microfluidics-based devices: New tools for studying cancer and cancer stem cell migration.Biomicrofluidics · 2011Article
- Stem cells in microfluidics.Biomicrofluidics · 2011Article
- Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device.Lab on a chip · 2010Article
- Article
- A gradient-generating microfluidic device for cell biology.Journal of visualized experiments : JoVE · 2007Article
- A microfluidic device with groove patterns for studying cellular behavior.Journal of visualized experiments : JoVE · 2007Article
- Amoeboid chemotaxis: future challenges and opportunities.Cell adhesion & migrationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundMicrofluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practical and technical limitations that impede wider implementation in routine biomedical research. Specialized equipment and protocols required for fabrication and setting up microfluidic experiments present hurdles for routine use by most biology laboratories.
resultsWe have developed and validated a novel microfluidic device that can directly interface with conventional tissue culture methods to generate and maintain controlled soluble environments in a Petri dish. It incorporates separate sets of fluidic channels and vacuum networks on a single device that allows reversible application of microfluidic gradients onto wet cell culture surfaces. Stable, precise concentration gradients of soluble factors were generated using simple microfluidic channels that were attached to a perfusion system. We successfully demonstrated real-time optical live/dead cell imaging of neural stem cells exposed to a hydrogen peroxide gradient and chemotaxis of metastatic breast cancer cells in a growth factor gradient.
conclusionThis paper describes the design and application of a versatile microfluidic device that can directly interface with conventional cell culture methods. This platform provides a simple yet versatile tool for incorporating the advantages of a microfluidic approach to biological assays without changing established tissue culture protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.